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Clin Transl Oncol. 2015 Nov;17(11):903-9. doi: 10.1007/s12094-015-1325-8. Epub 2015 Jun 24.

miR-448 negatively regulates ovarian cancer cell growth and metastasis by targeting CXCL12.

Author information

1
Department of Obstetrics, Shiyan Taihe Hospital, Hubei University of Medcine, 32 Renmin South Road, Shiyan, Hubei, China. lv_yuefeng@126.com.
2
Department of Obstetrics, Shiyan Taihe Hospital, Hubei University of Medcine, 32 Renmin South Road, Shiyan, Hubei, China.
3
Department of Gynaecology, Shiyan Taihe Hospital, Hubei University of Medicine, Shiyan, Hubei, China.

Abstract

OBJECTIVES:

The purpose of the study is to investigate the roles of miR-448 in ovarian cancer.

METHODS:

miR-448 and CXCL12 mRNA expression were examined using qRT-PCR. CXCL12 promoter activity was detected by luciferase activity system. Cell proliferation was assayed by MTT or colony formation. Migration and invasion was assayed by transwell chamber.

RESULTS:

miR-448 expression was usually under-expressed in ovarian cancer tissues and cell lines compared with their normal ones. Ectopic expression of miR-448 inhibited cell proliferation, migration and invasion in ovarian cancer cells. Moreover, bioinformatic prediction suggested that CXCL12 was a target gene of miR-448. We also demonstrated that restored expression of CXCL12 dampened miR-448-mediated suppression of tumor progression, which suggests the important role of miR-448 in tumor progression.

CONCLUSION:

Our data indicate that miR-448 functions as a tumor suppressor in ovarian cancer, which exerts its activity by suppressing the expression of CXCL12.

KEYWORDS:

CXCL12; Metastasis; Ovarian cancer; Proliferation; miR-448

PMID:
26103953
DOI:
10.1007/s12094-015-1325-8
[Indexed for MEDLINE]

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