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Can J Microbiol. 2015 Aug;61(8):597-601. doi: 10.1139/cjm-2015-0107. Epub 2015 May 29.

Virulence and antimicrobial susceptibility of clinical and environmental strains of Aeromonas spp. from northeastern Brazil.

Author information

1
a Department of Pathology and Legal Medicine, Postgraduate Program in Medical Microbiology, Federal University of Ceará, Fortaleza, Ceará, Brazil.
2
b School of Veterinary Medicine, Postgraduate Program in Veterinary Sciences, State University of Ceará, Fortaleza, Ceará, Brazil.
3
c Department of Pathology and Legal Medicine, Postgraduate Program in Medical Sciences, Federal University of Ceará, Fortaleza, Ceará, Brazil.
4
d Department of Statistics and Applied Mathematics, Federal University of Ceará, Fortaleza, Ceará, Brazil.
5
e School of Medicine, Christus College - Unichristus, Fortaleza, Ceará, Brazil.
6
f Diagnósticos da América SA (DASA), Fortaleza, Ceará, Brazil.

Abstract

The aims of the present study were to isolate and identify clinical and environmental strains of Aeromonas spp. by means of biochemical tests and the automated method VITEK 2 and to investigate the presence of the virulence genes cytotoxic enterotoxin (act), hemolysin (asa-1), and type III secretion system (ascV), and also the in vitro antimicrobial susceptibility of the strains. From the clinical isolates, 19 Aeromonas hydrophila, 3 Aeromonas veronii bv. sobria, and 1 Aeromonas caviae were identified, while from the environmental strains, 11 A. hydrophila, 22 A. veronii bv. sobria, 1 A. veronii bv. veronii, and 1 A. caviae were recovered. The gene act was detected in 69.5% of clinical isolates, asa-1 in 8.6%, and ascV in 34.7%. In the environmental strains, the detection rates were 51.4%, 45.7%, and 54.2% for the genes act, asa-1, and ascV, respectively. Resistance to amoxicillin-clavulanate and piperacillin-tazobactam was observed in 15 and 3 clinical strains, respectively, and resistance to ceftazidime, meropenem, imipenem, ciprofloxacin, and trimethoprim-sulfamethoxazole was observed in 1 strain for each drug. Resistance to amoxicillin-clavulanate and piperacillin-tazobactam was detected in 17 and 1 environmental strain, respectively. Higher resistance percentages were observed in clinical strains, but environmental strains also showed this phenomenon and presented a higher detection rate of virulence genes. Thus, it is important to monitor the antimicrobial susceptibility and pathogenic potential of the environmental isolates.

KEYWORDS:

Aeromonas spp.; antimicrobial resistance; environments; environnements; gènes de virulence; human infections; infections humaines; résistance antimicrobienne; virulence genes

PMID:
26103449
DOI:
10.1139/cjm-2015-0107
[Indexed for MEDLINE]

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