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Am J Cancer Res. 2015 Mar 15;5(4):1553-7. eCollection 2015.

Programmed cell death protein 1 and programmed death-ligand 1 are expressed on the surface of some small-cell lung cancer lines.

Author information

1
Department of General Internal Medicine 4, Kawasaki Medical School 2-1-80 Nakasange, Kita-ku, Okayama 700-8505, Japan.
2
Department of General Internal Medicine 4, Kawasaki Medical School 2-1-80 Nakasange, Kita-ku, Okayama 700-8505, Japan ; Department of Allergy and Respiratory Medicine (Thoracic Oncology), Okayama University Hospital 2-5-1, Shikata-cho, Kitaku, Okayama, 700-8558, Japan.
3
Department of Allergy and Respiratory Medicine (Thoracic Oncology), Okayama University Hospital 2-5-1, Shikata-cho, Kitaku, Okayama, 700-8558, Japan.

Abstract

INTRODUCTION:

Programmed cell death protein 1 (PD-1) and programmed death-ligand 1 (PD-L1) play a major role in suppressing the immune system during the formation of the PD-1/PD-L1 pathway, which transmits an inhibitory signal to reduce T cell activity. PD-L1 is often expressed in various malignant tumors. In contrast, PD-1 is generally observed in activated lymphocytes and myeloid-derived dendritic cells. Of the malignant cells, only Jurkat cells under special conditions and angioimmunoblastic T-cell lymphoma tissue cells express PD-1 on their surface.

METHODS:

To clarify whether the PD-1/PD-L1 pathway participates in the immunotolerance of small-cell lung cancer (SCLC) cells, we examined the expressions of PD-1 and PD-L1 on the cell surface of SCLC cell lines using flow cytometry and reverse transcription polymerase chain reaction.

RESULTS:

Among the four SCLC cell lines examined, only SBC-3 expressed both PD-1 and PD-L1.

CONCLUSIONS:

We demonstrated that both PD-1 and PD-L1 molecules were co-expressed on the surface of SCLC cells. Although the biological implications of this remain unclear, we speculate that PD-1 and its ligand on the SCLC cells may participate in the growth inhibition of tumor cells as reported in cytotoxic T cells.

KEYWORDS:

PD-1; PD-L1; Small-cell lung cancer; cell line; co-expression

PMID:
26101718
PMCID:
PMC4473331

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