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J Ethnopharmacol. 2015 Aug 22;172:232-7. doi: 10.1016/j.jep.2015.05.060. Epub 2015 Jun 20.

Trichilia catigua ethyl-acetate fraction protects against cognitive impairments and hippocampal cell death induced by bilateral common carotid occlusion in mice.

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  • 1Laboratory of Neuropsychopharmacology, Department of Pharmacology and Therapeutics, Maringá 87020-900, Paraná, Brazil.
  • 2Laboratory of Pharmaceutical Biology, Palafito, Maringá, Brazil.
  • 3Laboratory of Microbiology, Universidade Estadual de Maringá, Av. Colombo, 5790, Maringá 87020-900, Paraná, Brazil.
  • 4Laboratory of Neuropsychopharmacology, Department of Pharmacology and Therapeutics, Maringá 87020-900, Paraná, Brazil. Electronic address:



Trichilia catigua preparations have antinociceptive, antiinflammatory, and neuroprotective activity. Recently, a neuroprotective role for T. catigua was proposed using an in vitro model of ischemia-reperfusion in rat hippocampal slices. The aim of the present study was to evaluate the effects of an ethyl-acetate fraction (EAF) of T. catigua, which has potent antioxidant activity, in mice subjected to an in vivo model of cerebral ischemia.


Male Swiss mice were subject to the bilateral common carotid occlusion (BCCAO) model of cerebral ischemia. The animals were orally administered the T. catigua EAF (200, 400, or 800 mg/kg) 30 min before and once per day for 7 days after BCCAO. Histological and behavioral outcomes were assessed using Nissl staining and the Morris water maze test of cognition, respectively.


Mice that were subjected to BCCAO exhibited cognitive impairments in the Morris water maze. The spatial cognitive deficits were counteracted by T. catigua EAF administration (200-800 mg/kg). The T. catigua EAF significantly increased the number of intact-appearing Nissl-stained cells in the hippocampus in BCCAO mice.


These results show that the T. catigua EAF promoted functional recovery, decreased the delayed hippocampal cell loss, and mitigated the ongoing neurodegenerative processes induced by BCCAO in mice.


ANOVA=analysis of variance; BCCAO=bilateral common carotid occlusion; Bilateral common carotid occlusion; CE=crude extract; Cognition; DPPH=2,2-diphenyl-1-picryl-hydrazyl; EAF=Trichilia catigua ethyl-acetate fraction; EPC=epicatechin; HPLC=high performance liquid chromatography; Morris water maze; Neurodegeneration; OGD=oxygen and glucose deprivation; PB2=procyanidin B2; ROS=reactive oxygen species; SOD=superoxide dismutase; TFA=trifluoroacetic acid; TGCI=transient global cerebral ischemia; Trichilia catigua ethyl-acetate fraction

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