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Int J Clin Exp Pathol. 2015 Apr 1;8(4):3451-9. eCollection 2015.

Anti-inflammatory and antioxidant effects of curcumin on acute lung injury in a rodent model of intestinal ischemia reperfusion by inhibiting the pathway of NF-Kb.

Author information

1
Department of General Surgery, The Third People's Hospital of Dalian Dalian 116033, China.
2
Department of Pharmacology, Dalian Medical University Dalian 116044, China.
3
Department of General Surgery, Liaoning Cancer Hospital and Institute Shenyang 110042, China.
4
Department of General Surgery, The Second Hospital of Dalian Medical University Dalian 116023, China.

Abstract

OBJECTIVE:

To investigate the anti-inflammatory and antioxidant effect of curcumin on lung lesion induced by intestinal ischemia reperfusion injury (IIR).

METHODS:

Rats were divided into four groups: sham, intestinal IIR (IIR), 1 mg/kg of curcumin treatment group (1 mg/kg), and 5 mg/kg of curcumin treatment group (5 mg/kg). Curcumin was given respectively (1 mg/kg and 5 mg/kg) following the above doses. IIR was produced by 1 h of intestinal ischemia followed by 2 h of reperfusion. Rats were sacrificed at the end of reperfusion and lung tissues were collected for biochemical and histopathological examination in 4 groups. Lung tissues histology and bronchoalveolar lavage fluid (BALF) protein were assayed. Serum IL-6, lung superoxide dismutase (SOD) and myeloperoxidase (MPO) were measured. The expression level of NF-κB and ICAM-1 (including immunohistochemical analysis and western blot analysis) were also measured.

RESULTS:

Lung tissue injury induced by IIR was obviously observed through pathology and BALF protein. MPO activity, IL-6 level and ICAM-1 expression were significantly increased with the elevation of NF-κB, simultaneously, SOD activity was decreased. With Treatment of curcumin, pathology and BALF protein of lung tissue were improved clearly. Inflammatory indexes (MPO activity, IL-6 level and ICAM-1) were improved and antioxidant index (SOD activity) was enhanced paralleled with NF-κB.

CONCLUSION:

Using curcumin effectively prevented IIR-induced lung injury. Anti-inflammatory and antioxidant effects of curcumin could be observed by inhibiting the pathway of NF-κB.

KEYWORDS:

Curcumin; NF-κB; intestinal ischemia reperfusion; lung injury

PMID:
26097529
PMCID:
PMC4466916
[Indexed for MEDLINE]
Free PMC Article

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