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Biotechnol Prog. 2015 Sep-Oct;31(5):1331-9. doi: 10.1002/btpr.2135. Epub 2015 Jul 15.

Characterizing the release of bioactive N-glycans from dairy products by a novel endo-β-N-acetylglucosaminidase.

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Dept. of Food Science and Technology, University of California, One Shields Avenue, Davis, CA, 95616.
Foods for Health Institute, University of California, One Shields Avenue, Davis, CA, 95616.
Dept. of Viticulture and Enology, University of California, Davis, CA, 95616.
Dept. of Chemical Engineering and Materials Science, University of California, Davis, CA, 95616.
Foods for Health Inst., University of California, One Shields Avenue, Davis, CA, 95616.


Endo-β-N-acetylglucosaminidase isolated from B. infantis ATCC 15697 (EndoBI-1) is a novel enzyme that cleaves N-N'-diacetyl chitobiose moieties found in the N-glycan core of high mannose, hybrid, and complex N-glycans. These conjugated N-glycans are recently shown as a new prebiotic source that stimulates the growth of a key infant gut microbe, Bifidobacterium longum subsp. Infantis. The effects of pH (4.45-8.45), temperature (27.5-77.5°C), reaction time (15-475 min), and enzyme/protein ratio (1:3,000-1:333) were evaluated on the release of N-glycans from bovine colostrum whey by EndoBI-1. A central composite design was used, including a two-level factorial design (2(4)) with four center points and eight axial points. In general, low pH values, longer reaction times, higher enzyme/protein ratio, and temperatures around 52°C resulted in the highest yield. The results demonstrated that bovine colostrum whey, considered to be a by/waste product, can be used as a glycan source with a yield of 20 mg N-glycan/g total protein under optimal conditions for the ranges investigated. Importantly, these processing conditions are suitable to be incorporated into routine dairy processing activities, opening the door for an entirely new class of products (released bioactive glycans and glycan-free milk). The new enzyme's activity was also compared with a commercially available enzyme, showing that EndoBI-1 is more active on native proteins than PNGase F and can be efficiently used during pasteurization, streamlining its integration into existing processing strategies.


N-glycans; endo-β-N-acetylglucosaminidase; whey

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