Int J Mol Sci. 2015 Jun 18;16(6):13959-72. doi: 10.3390/ijms160613959.

Transcriptional Profiling Reveals Differential Gene Expression of Amur Ide (Leuciscus waleckii) during Spawning Migration.

Cui J^1,², Xu J^3,⁴, Zhang S⁵, Wang K⁶, Jiang Y^7,⁸, Mahboob S^9,¹⁰, Al-Ghanim KA¹¹, Xu P^12,¹³.

Author information

1: Chinese Academy of Fishery Sciences Key Laboratory of Aquatic Genomics, Centre for Applied Aquatic Genomics, Chinese Academy of Fishery Sciences, Beijing 100141, China. cuijun@mail.dlut.edu.cn.
2: Beijing Key Laboratory of Fishery Biotechnology, Beijing 100141, China. cuijun@mail.dlut.edu.cn.
3: Chinese Academy of Fishery Sciences Key Laboratory of Aquatic Genomics, Centre for Applied Aquatic Genomics, Chinese Academy of Fishery Sciences, Beijing 100141, China. xuj@cafs.ac.cn.
4: Beijing Key Laboratory of Fishery Biotechnology, Beijing 100141, China. xuj@cafs.ac.cn.
5: Chinese Academy of Fishery Sciences Key Laboratory of Aquatic Genomics, Centre for Applied Aquatic Genomics, Chinese Academy of Fishery Sciences, Beijing 100141, China. zsh891007@gmail.com.
6: Chinese Academy of Fishery Sciences Key Laboratory of Aquatic Genomics, Centre for Applied Aquatic Genomics, Chinese Academy of Fishery Sciences, Beijing 100141, China. wangk@cafs.ac.cn.
7: Chinese Academy of Fishery Sciences Key Laboratory of Aquatic Genomics, Centre for Applied Aquatic Genomics, Chinese Academy of Fishery Sciences, Beijing 100141, China. jiangyl@cafs.ac.cn.
8: Beijing Key Laboratory of Fishery Biotechnology, Beijing 100141, China. jiangyl@cafs.ac.cn.
9: Department of Zoology, College of Science, King Saud University, P.O. Box 2455, Riyadh 11451, Saudi Arabia. mushahid@ksu.edu.sa.
10: Department of Zoology, GC University, Faisalabad 38030, Pakistan. mushahid@ksu.edu.sa.
11: Department of Zoology, College of Science, King Saud University, P.O. Box 2455, Riyadh 11451, Saudi Arabia. kghanim@ksu.edu.sa.
12: Chinese Academy of Fishery Sciences Key Laboratory of Aquatic Genomics, Centre for Applied Aquatic Genomics, Chinese Academy of Fishery Sciences, Beijing 100141, China. xupeng@cafs.ac.cn.
13: Beijing Key Laboratory of Fishery Biotechnology, Beijing 100141, China. xupeng@cafs.ac.cn.

Abstract

Amur ide (Leuciscus waleckii), an important aquaculture species, inhabits neutral freshwater but can tolerate high salinity or alkalinity. As an extreme example, the population in Dali Nor lake inhabits alkalized soda water permanently, and migrates from alkaline water to neutral freshwater to spawn. In this study, we performed comparative transcriptome profiling study on the livers of Amur ide to interrogate the expression differences between the population that permanently inhabit freshwater in Ganggeng Nor lake (FW) and the spawning population that recently migrated from alkaline water into freshwater (SM). A total of 637,234,880 reads were generated, resulting in 53,440 assembled contigs that were used as reference sequences. Comparisons of these transcriptome files revealed 444 unigenes with significant differential expression (p-value ≤ 0.01, fold-change ≥ 2), including 246 genes that were up-regulated in SM and 198 genes that were up-regulated in FW. The gene ontology (GO) enrichment analysis and KEGG pathway analysis indicated that the mTOR signaling pathway, Janus kinase-signal transducer and activator of transcription (JAK-STAT) signaling pathway, and oxidative phosphorylation were highly likely to affect physiological changes during spawning migration. Overall, this study demonstrates that transcriptome changes played a role in Amur ide spawning migration. These results provide a foundation for further analyses on the physiological and molecular mechanisms underlying Amur ide spawning migration.