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Cell Rep. 2015 Jun 30;11(12):1856-65. doi: 10.1016/j.celrep.2015.05.033. Epub 2015 Jun 18.

Fasting, but Not Aging, Dramatically Alters the Redox Status of Cysteine Residues on Proteins in Drosophila melanogaster.

Author information

1
MRC Mitochondrial Biology Unit, Cambridge CB2 0XY, UK; Institute of Ophthalmology, University College London, London EC1V 9EL, UK.
2
MRC Mitochondrial Biology Unit, Cambridge CB2 0XY, UK.
3
Institute of Healthy Ageing and GEE, University College London, London WC1E 6BT, UK; Max Planck Institute for Biology of Ageing, Cologne 50931, Germany; MRC Clinical Sciences Centre, Imperial College London, London W12 0NN, UK.
4
MRC Mitochondrial Biology Unit, Cambridge CB2 0XY, UK; Department of Medicine, University of Cambridge, Cambridge CB2 0QQ, UK; Department of Cancer Biology, Dana-Farber Cancer Institute, Boston, MA 02215, USA; Department of Cell Biology, Harvard Medical School, Boston, MA 02115-5730, USA.
5
Institute of Healthy Ageing and GEE, University College London, London WC1E 6BT, UK; Max Planck Institute for Biology of Ageing, Cologne 50931, Germany.
6
MRC Mitochondrial Biology Unit, Cambridge CB2 0XY, UK. Electronic address: mpm@mrc-mbu.cam.ac.uk.

Abstract

Altering the redox state of cysteine residues on protein surfaces is an important response to environmental challenges. Although aging and fasting alter many redox processes, the role of cysteine residues is uncertain. To address this, we used a redox proteomic technique, oxidative isotope-coded affinity tags (OxICAT), to assess cysteine-residue redox changes in Drosophila melanogaster during aging and fasting. This approach enabled us to simultaneously identify and quantify the redox state of several hundred cysteine residues in vivo. Cysteine residues within young flies had a bimodal distribution with peaks at ∼10% and ∼85% reversibly oxidized. Surprisingly, these cysteine residues did not become more oxidized with age. In contrast, 24 hr of fasting dramatically oxidized cysteine residues that were reduced under fed conditions while also reducing cysteine residues that were initially oxidized. We conclude that fasting, but not aging, dramatically alters cysteine-residue redox status in D. melanogaster.

PMID:
26095360
PMCID:
PMC4508341
DOI:
10.1016/j.celrep.2015.05.033
[Indexed for MEDLINE]
Free PMC Article
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