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Mar Genomics. 2015 Dec;24 Pt 3:229-30. doi: 10.1016/j.margen.2015.06.002. Epub 2015 Jun 18.

Complete genome sequence of the hyperthermophilic methanogen Methanocaldococcus bathoardescens JH146(T) isolated from the basalt subseafloor.

Author information

1
Graduate School of Biotechnology, Institute of Life Science and Resources, Kyung Hee University, Yongin 446-701, Republic of Korea.
2
Graduate School of Biotechnology, Institute of Life Science and Resources, Kyung Hee University, Yongin 446-701, Republic of Korea; Research Division for Biotechnology, Korea Atomic Energy Research Institute, Jeongeup 580-185, Republic of Korea.
3
Department of Microbiology, University of Massachusetts, Amherst, MA 01003, USA.
4
Agricultural Microbiology Division, National Academy of Agricultural Science, Suwon 441-707, Republic of Korea.
5
Graduate School of Biotechnology, Institute of Life Science and Resources, Kyung Hee University, Yongin 446-701, Republic of Korea. Electronic address: cspark@khu.ac.kr.

Abstract

Methanocaldococcus bathoardescens JH146(T) is a hyperthermophilic and obligate hydrogenotrophic methanogen isolated from low-temperature (26 °C) hydrothermal vent fluid at Axial Seamount in the northeastern Pacific Ocean. It is most closely related to the N2-fixing methanogen Methanocaldococcus sp. FS406-22; however, they differ in that JH146 cannot fix N2 or reductively assimilate nitrate. In this study, we present the complete genome sequence of strain JH146(T) (1,607,556 bp) with its 1635 protein coding genes, and 41 RNA genes. Our analysis focuses on its methane production via the acetyl-CoA pathway and its deleted gene clusters related to nitrogen assimilation. This study extends our understanding of methanogenesis at high temperatures and the impact of these organisms on the biogeochemistry of subseafloor hydrothermal environments and the deep sea.

KEYWORDS:

Genome; Hyperthermophile; Methanocaldococcus sp.; Methanogenesis

PMID:
26094864
DOI:
10.1016/j.margen.2015.06.002
[Indexed for MEDLINE]

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