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Mol Biochem Parasitol. 2015 May;201(1):76-82. doi: 10.1016/j.molbiopara.2015.06.001. Epub 2015 Jun 18.

Identification of the minimal binding region of a Plasmodium falciparum IgM binding PfEMP1 domain.

Author information

1
Institute of Immunology and Infection Research, Centre for Immunity, Infection and Evolution, School of Biological Sciences, University of Edinburgh, Edinburgh EH9 3FL, United Kingdom.
2
Bio-ID Center, School of Biomedical Engineering, Shanghai Jiao Tong University, Shanghai, China.
3
Department of Infection, Immunity and Inflammation, University of Leicester, Leicester, United Kingdom.
4
Clinical Sciences Research Laboratories, Warwick Medical School, Coventry CV2 2DX, United Kingdom.
5
Institute of Immunology and Infection Research, Centre for Immunity, Infection and Evolution, School of Biological Sciences, University of Edinburgh, Edinburgh EH9 3FL, United Kingdom. Electronic address: Alex.Rowe@ed.ac.uk.

Abstract

Binding of host immunoglobulin is a common immune evasion mechanism demonstrated by microbial pathogens. Previous work showed that the malaria parasite Plasmodium falciparum binds the Fc-region of human IgM molecules, resulting in a coating of IgM on the surface of infected erythrocytes. IgM binding is a property of P. falciparum strains showing virulence-related phenotypes such as erythrocyte rosetting. The parasite ligands for IgM binding are members of the diverse P. falciparum Erythrocyte Membrane Protein One (PfEMP1) family. However, little is known about the amino acid sequence requirements for IgM binding. Here we studied an IgM binding domain from a rosette-mediating PfEMP1 variant, DBL4ζ of TM284var1, and found that the minimal IgM binding region mapped to the central region of the DBL domain, comprising all of subdomain 2 and adjoining parts of subdomains 1 and 3. Site-directed mutagenesis of charged amino acids within subdomain 2, predicted by molecular modelling to form the IgM binding site, showed no marked effect on IgM binding properties. Overall, this study identifies the minimal IgM binding region of a PfEMP1 domain, and indicates that the existing homology model of PfEMP1-IgM interaction is incorrect. Further work is needed to identify the specific interaction site for IgM within the minimal binding region of PfEMP1.

KEYWORDS:

Cell Adhesion; DBL domain; Fc-receptor; Immunoglobulin M; Rosetting; Var gene

PMID:
26094597
PMCID:
PMC4539346
DOI:
10.1016/j.molbiopara.2015.06.001
[Indexed for MEDLINE]
Free PMC Article

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