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Mol Cell Neurosci. 2015 Sep;68:177-85. doi: 10.1016/j.mcn.2015.06.008. Epub 2015 Jun 16.

Profiles of secreted neuropeptides and catecholamines illustrate similarities and differences in response to stimulation by distinct secretagogues.

Author information

1
Skaggs School of Pharmacy and Pharmaceutical Sciences, Univ. of Calif.-San Diego, La Jolla, CA 92093, United States.
2
Dept. of Medicine, Univ. of Calif.-San Diego, La Jolla, CA 92093, United States.
3
Skaggs School of Pharmacy and Pharmaceutical Sciences, Univ. of Calif.-San Diego, La Jolla, CA 92093, United States; Dept. of Neuroscience and Dept. of Pharmacology, School of Medicine, Univ. of Calif.-San Diego, La Jolla, CA 92093, United States. Electronic address: vhook@ucsd.edu.

Abstract

The goal of this study was to define profiles of secreted neuropeptide and catecholamine neurotransmitters that undergo co-release from sympathoadrenal chromaffin cells upon stimulation by distinct secretagogues. Chromaffin cells of the adrenal medulla participate in the dynamic responses to stress, especially that of 'fight and flight', and, thus, analyses of the co-release of multiple neurotransmitters is necessary to gain knowledge of how the stress response regulates cell-cell communication among physiological systems. Results of this study demonstrated that six different secretagogues stimulated the co-release of the neuropeptides Met-enkephalin, galanin, NPY, and VIP with the catecholamines dopamine, norepinephrine, and epinephrine. Importantly, the quantitative profiles of the secreted neurotransmitters showed similarities and differences upon stimulation by the different secretagogues evaluated, composed of KCl depolarization, nicotine, carbachol, PACAP, bradykinin, and histamine. The rank-orders of the secreted profiles of the neurotransmitters were generally similar among these secretagogues, but differences in the secreted amounts of each neurotransmitter occurred with different secretagogues. Epinephrine among the catecholamines showed the highest level of secretion. (Met)enkephalin showed the largest levels of secretion compared to the other neuropeptides examined. Levels of secreted catecholamines were greater than that of the neuropeptides. These data support the hypothesis that profiles of secreted neuropeptide and catecholamine neurotransmitters show similarities and differences upon stimulation by distinct secretagogues. These results illustrate the co-release of concerted neurotransmitter profiles that participate in the stress response of the sympathoadrenal nervous system.

KEYWORDS:

Catecholamines; Chromaffin cells; Neuropeptides; Neurotransmitter profiles; Secretagogues; Secretion; Stress response

PMID:
26092702
PMCID:
PMC4593749
DOI:
10.1016/j.mcn.2015.06.008
[Indexed for MEDLINE]
Free PMC Article

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