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Int J Biol Macromol. 2015 Sep;80:23-8. doi: 10.1016/j.ijbiomac.2015.05.035. Epub 2015 Jun 16.

Anticancer properties of low molecular weight oat beta-glucan – An in vitro study.

Author information

1
Department of Medical Biochemistry, Wroclaw Medical University, Chalubinskiego 10, 50-368 Wroclaw, Poland. Electronic address: anna.choromanska@umed.wroc.pl.
2
Department of Medical Biochemistry, Wroclaw Medical University, Chalubinskiego 10, 50-368 Wroclaw, Poland.
3
Department of Food Biotechnology, Wroclaw University of Economics, Komandorska 118-120, 53-345 Wroclaw, Poland.

Abstract

Anticancer properties of 1-3, 1-4 oat beta glucan are under intensive investigation now. Antitumor characteristic of fungi and yeast beta-glucans have been widely recognized, but those polysaccharides are mostly insoluble which creates several problems especially in topical formulation. Also high molecular weight oat beta-glucans reveal high viscosity which restricts its application. According to those problems in the current study the antitumor activities of low molecular weight beta-glucan derived from oats were investigated in cancer cells: Me45, A431 and normal HaCaT and murine macrophages P388/D1. The low molecular weight beta-glucan from oat significantly deceased cancer cells viability, while for the normal cells it was non-toxic. It was observed that with the increasing incubation time and the beta-glucan concentration the cancer cells viability significantly deceased. Furthermore for the normal cells the low molecular weight beta-glucan from oat was non-toxic. Immunocytochemical ABC analysis showed that beta-glucan induced strong expression of caspase-12 in both cancer cell lines, while in HaCaT cells ABC reaction was significantly lower and in P388/D1 cell line ABC reaction was negative. Our preliminary studies show strong anti-tumor properties of new low molecular weight beta-glucan from oat and at the same time no toxicity for normal cells.

KEYWORDS:

Melanoma; Oat beta-glucan; Skin cancer

PMID:
26092171
DOI:
10.1016/j.ijbiomac.2015.05.035
[Indexed for MEDLINE]

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