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Med Mycol. 2016 May;54(4):428-32. doi: 10.1093/mmy/myv036. Epub 2015 Jun 19.

Combination of fluconazole with silver nanoparticles produced by Fusarium oxysporum improves antifungal effect against planktonic cells and biofilm of drug-resistant Candida albicans.

Author information

1
Programa de Pós-Graduação em Microbiologia, Universidade Estadual de Londrina, Londrina, PR, Brazil lionilmy@uel.br.
2
Departamento de Microbiologia, Centro de Ciências Biológicas, Universidade Estadual de Londrina, Londrina, PR, Brazil lionilmy@uel.br.
3
Departamento de Microbiologia, Centro de Ciências Biológicas, Universidade Estadual de Londrina, Londrina, PR, Brazil Programa Nacional de Pós Doutorado (PNPD), Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES), Brazil.
4
Faculdade de Ciências Farmacêuticas de Ribeirão Preto, Universidade de São Paulo, Ribeirão Preto, SP, Brazil.
5
Instituto de Química, Universidade Estadual de Campinas, Campinas, SP, Brazil.
6
Departamento de Ciências Patológicas, Centro de Ciências Biológicas, Universidade Estadual de Londrina, Londrina, PR, Brazil.
7
Programa de Pós-Graduação em Microbiologia, Universidade Estadual de Londrina, Londrina, PR, Brazil Departamento de Microbiologia, Centro de Ciências Biológicas, Universidade Estadual de Londrina, Londrina, PR, Brazil.

Abstract

Silver nanoparticles (AgNPs) have been extensively studied because of their anti-microbial potential. Here, we evaluated the effect of biologically synthesized silver nanoparticles (AgNPbio) alone and in combination with fluconazole (FLC) against planktonic cells and biofilms of FLC-resistant Candida albicans AgNPbio exhibited a fungicidal effect, with a minimal inhibitory concentration (MIC) and fungicidal concentration ranging from 2.17 to 4.35 μg/ml. The combination of AgNPbio and FLC reduced the MIC of FLC around 16 to 64 times against planktonic cells of allC. albicans There was no significant inhibitory effect of AgNPbio on biofilm cells. However, FLC combined with AgNPbio caused a significant dose-dependent decrease in the viability of both initial and mature biofilm. All concentrations of AgNPbio, alone or in combination with FLC, were not cytotoxic to mammalian cells.The results highlight the effectiveness of the combination of AgNPbio with FLC against FLC-resistant C. albicans.

KEYWORDS:

biological nanoparticles; fluconazole resistance; fungicidal effect

PMID:
26092103
DOI:
10.1093/mmy/myv036
[Indexed for MEDLINE]

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