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J Virol Methods. 2015 Sep 15;222:110-6. doi: 10.1016/j.jviromet.2015.06.006. Epub 2015 Jun 16.

Development and characterization of polyclonal peptide antibodies for the detection of Yellow fever virus proteins.

Author information

1
Centre for Biological Threats and Special Pathogens (ZBS1), Robert Koch-Institut, Nordufer 20, 13353 Berlin, Germany; The National Institute of Public Health, Prague, Czech Republic; European Program for Public Health Microbiology (EUPHEM), ECDC, Stockholm, Sweden. Electronic address: nkstock2015@gmail.com.
2
Centre for Biological Threats and Special Pathogens (ZBS1), Robert Koch-Institut, Nordufer 20, 13353 Berlin, Germany; Institut Pasteur, Environment and Infectious Risks Research and Expertise Unit, Laboratory for Urgent Response to Biological Threats, Paris, France.
3
Centre for Biological Threats and Special Pathogens (ZBS1), Robert Koch-Institut, Nordufer 20, 13353 Berlin, Germany; Freie Universität, Berlin, Germany.
4
Centre for Biological Threats and Special Pathogens (ZBS1), Robert Koch-Institut, Nordufer 20, 13353 Berlin, Germany; Université Montpellier 2, Montpellier, France.
5
Centre for Biological Threats and Special Pathogens (ZBS1), Robert Koch-Institut, Nordufer 20, 13353 Berlin, Germany.

Abstract

There is still a considerable need for development of new tools and methods detecting specific viral proteins for the diagnosis and pathogenesis study of the Yellow fever virus (YFV). This study aimed to develop and characterize polyclonal peptide antisera for detection of YFV-C and -NS1 proteins. The antisera were used further to investigate NS1 protein expression during YFV infection in mammalian cells. YFV target proteins were detected by all antisera in western blot and immunofluorescence assays. No cross-reactivity was observed with Dengue virus, West Nile virus, Tick-borne encephalitis virus and Japanese encephalitis virus. Nuclear localization of the YFV-C protein was demonstrated for the first time. Experiments investigating NS1 expression suggested a potential use of the YFV-NS1 antisera for development of diagnostic approaches targeting the secreted form of the NS1 protein. The antisera described in this study offer new possibilities for use in YFV research and for the development of novel diagnostic tests.

KEYWORDS:

Capsid protein; NS1 protein; Peptide antisera; Yellow fever virus

PMID:
26086983
DOI:
10.1016/j.jviromet.2015.06.006
[Indexed for MEDLINE]

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