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Br J Nutr. 2015 Jul;114(2):220-30. doi: 10.1017/S0007114515001750. Epub 2015 Jun 17.

Butyrylated starch intake can prevent red meat-induced O6-methyl-2-deoxyguanosine adducts in human rectal tissue: a randomised clinical trial.

Author information

1
Commonwealth Scientific and Industrial Research Organisation (CSIRO), Food and Nutrition Flagship,PO Box 10041,Adelaide BC,SA5000,Australia.
2
Flinders Centre for Innovation in Cancer, Flinders University of South Australia,Bedford Park,SA5042,Australia.
3
Rowett Institute of Nutrition and Health, University of Aberdeen,AberdeenAB21 9SB,UK.
4
Commonwealth Scientific and Industrial Research Organisation Mathematics, Informatics and Statistics,Glen Osmond,SA5064,Australia.

Abstract

Epidemiological studies have identified increased colorectal cancer (CRC) risk with high red meat (HRM) intakes, whereas dietary fibre intake appears to be protective. In the present study, we examined whether a HRM diet increased rectal O(6)-methyl-2-deoxyguanosine (O(6)MeG) adduct levels in healthy human subjects, and whether butyrylated high-amylose maize starch (HAMSB) was protective. A group of twenty-three individuals consumed 300 g/d of cooked red meat without (HRM diet) or with 40 g/d of HAMSB (HRM+HAMSB diet) over 4-week periods separated by a 4-week washout in a randomised cross-over design. Stool and rectal biopsy samples were collected for biochemical, microbial and immunohistochemical analyses at baseline and at the end of each 4-week intervention period. The HRM diet increased rectal O(6)MeG adducts relative to its baseline by 21% (P < 0.01), whereas the addition of HAMSB to the HRM diet prevented this increase. Epithelial proliferation increased with both the HRM (P < 0.001) and HRM + HAMSB (P < 0.05) diets when compared with their respective baseline levels, but was lower following the HRM + HAMSB diet compared with the HRM diet (P < 0.05). Relative to its baseline, the HRM + HAMSB diet increased the excretion of SCFA by over 20% (P < 0.05) and increased the absolute abundances of the Clostridium coccoides group (P < 0.05), the Clostridium leptum group (P < 0.05), Lactobacillus spp. (P < 0.01), Parabacteroides distasonis (P < 0.001) and Ruminococcus bromii (P < 0.05), but lowered Ruminococcus torques (P < 0.05) and the proportions of Ruminococcus gnavus, Ruminococcus torques and Escherichia coli (P < 0.01). HRM consumption could increase the risk of CRC through increased formation of colorectal epithelial O(6)MeG adducts. HAMSB consumption prevented red meat-induced adduct formation, which may be associated with increased stool SCFA levels and/or changes in the microbiota composition.

KEYWORDS:

Butyrate; DNA adducts; Fermentation; Microbiota; Red meat; Resistant starch; SCFA

PMID:
26084032
PMCID:
PMC4531472
DOI:
10.1017/S0007114515001750
[Indexed for MEDLINE]
Free PMC Article

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