(a–d) Distribution of (a) GATA1, (b) GATA2, (c) actin and (d) CTCF fluorescence observed by flow cytometry. Distributions in grey depict isotype controls. (e) Bi-clustered heat map of single cell deviations as observed within K562 cells (N=239). Labels on right identify co-clustering of related factors. (f) Bi-clustered heat map of single-cell deviations observed from permuted data. (g) Projection of factor loadings onto principal component 1 versus 5 from principal component (PC) analysis of heatmap from . Factor loadings do not vary along PC5, while peaks associated with regions with different replication timings (RepliSeq) have strong variation along this axis. Venn-diagrams showing variability of (h) GATA1 and/or GATA2, (i) CJUN and/or GATA2 and CEBPB and/or GATA2 (co-) occurring ChIP-seq sites. (j) -log10(p-values) of calculated changes in co-occurring ChIP-seq sites shown in . (k) Distribution of accessibility among GATA1 only, GATA2 only, and shared sites. (l) Mean accessibility from GATA1 only, GATA2 only, and shared sites in (k), error bars represent 1 standard deviation generated by bootstrapping ChIP-seq peaks.