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Zhonghua Yi Xue Za Zhi. 2015 Apr 28;95(16):1214-7.

[Effects of chronic intermittent hypoxia on regulation of miRNA-214 in myocardial apoptosis in rats].

[Article in Chinese]

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Department of Pneumology, Affiliated People's Hospital, Fujian University of Traditional Chinese Medicine, Fuzhou 350000, China.
Department of Pneumology, First Affiliated Hospital, Fujian Medical University, Laboratory of Pneumology, Fujian Medical University, Fujian Provincial Sleep-Disordered Breathing Clinic Center, Fuzhou 350000, China; Email:



To observe the effects of chronic intermittent hypoxia (CIH) on myocardial cell apoptosis and explore the mechanism of microRNA-214 (miRNA-214) expression for myocardial cell apoptosis and cardiac dysfunction.


A total of 20 adult male Sprague-Dawley rats were randomized into two groups (n = 10 each): for CIH group, the rats were raised in a CIH chamber 8 h daily for 6 weeks; for normal control (NC) group, the animal were exposed to normoxic air 7 h daily for 6 weeks. Hemodynamic values and myocardial function were measured via a cannula inserted into right common carotid artery. Myocardial cell apoptosis was determined by terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL) method. The expressions of miRNA-214 and hypoxia-inducible factor-1α (HIF-1α) mRNA were observed by real-time polymerase chain reaction (PCR). The expressions of such apoptosis-related proteins as fatty acid synthase (Fas), caspase 3, caspase 8, B-cell lymphoma-2 (Bcl-2) and Bcl-2-Associated X Protein (Bax) were detected by Western blot.


Compared with NC group, left ventricle end diastolic pressure (LVEDP) ((4.1 ± 0.5) vs (2.7 ± 0.4) mmHg, 1 mmHg = 0.133 kPa) increased, maximal rate of pressure decline (-dp/dtmax) ((3 005 ± 86) vs (5 918 ± 112) mmHg/s), maximal rate of pressure development (+dp/dtmax) ((4 118 ± 76) vs (7 547 ± 271) mmHg/s) and left ventricular systolic pressure (LVSP) ((90.7 ± 2.5) vs (132.7 ± 5.5) mmHg] decreased in CIH group (all P < 0.001). There were significantly more TUNEL positive cells in CIH group versus NC group (P < 0.001). The expression of miRNA-214 was significantly lower in CIH group than that in NC group (P < 0.001). In addition, HIF-1α mRNA level was significantly higher in CIH group than that in NC group (P < 0.001). The expressions of caspase 3, caspase 8, Fas and Bax increased significantly in CIH group versus NC group (all P < 0.001), suggesting increased apoptosis of myocardial cells in CIH group. Compared with NC group, the expression of Bcl-2 decreased significantly in CIH group (P < 0.001).


CIH may decrease the hemodynamic values and myocardial function in rats. The expression of miRNA-214 in CIH group is significantly depressed through accelerated apoptosis of myocardial cells.

[Indexed for MEDLINE]

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