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Genetics. 2015 Aug;200(4):1219-28. doi: 10.1534/genetics.115.178053. Epub 2015 Jun 16.

Nitric Oxide Synthase Regulates Growth Coordination During Drosophila melanogaster Imaginal Disc Regeneration.

Author information

1
Department of Cell Biology, University of Virginia School of Medicine, Charlottesville, Virginia 22908.
2
Department of Cell Biology, University of Virginia School of Medicine, Charlottesville, Virginia 22908 ajh6a@virginia.edu.

Abstract

Mechanisms that coordinate growth during development are essential for producing animals with proper organ proportion. Here we describe a pathway through which tissues communicate to coordinate growth. During Drosophila melanogaster larval development, damage to imaginal discs activates a regeneration checkpoint through expression of Dilp8. This both produces a delay in developmental timing and slows the growth of undamaged tissues, coordinating regeneration of the damaged tissue with developmental progression and overall growth. Here we demonstrate that Dilp8-dependent growth coordination between regenerating and undamaged tissues, but not developmental delay, requires the activity of nitric oxide synthase (NOS) in the prothoracic gland. NOS limits the growth of undamaged tissues by reducing ecdysone biosynthesis, a requirement for imaginal disc growth during both the regenerative checkpoint and normal development. Therefore, NOS activity in the prothoracic gland coordinates tissue growth through regulation of endocrine signals.

KEYWORDS:

allometry; ecdysone; growth control; nitric oxide; regeneration

PMID:
26081194
PMCID:
PMC4574233
DOI:
10.1534/genetics.115.178053
[Indexed for MEDLINE]
Free PMC Article

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