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Fitoterapia. 2015 Sep;105:73-82. doi: 10.1016/j.fitote.2015.06.009. Epub 2015 Jun 14.

Chokeberry (Aronia melanocarpa (Michx.) Elliot) concentrate inhibits NF-κB and synergizes with selenium to inhibit the release of pro-inflammatory mediators in macrophages.

Author information

1
VivaCell Biotechnology GmbH, Denzlingen, Germany.
2
Ursapharm Arzneimittel GmbH, Saarbrücken, Germany.
3
IMIBIC, Reina Sofía University Hospital, University of Córdoba, Dept of Cell Biology, Physiology and Immunology, Avda Menéndez Pidal s/n 14004, Córdoba, Spain.
4
Institute of Food Science and Biotechnology, University of Hohenheim, Stuttgart, Germany.
5
Institute of Food Science and Biotechnology, University of Hohenheim, Stuttgart, Germany; Biological Science Department, King Abdulaziz University, Jeddah, Saudi Arabia.
6
IMIBIC, Reina Sofía University Hospital, University of Córdoba, Dept of Cell Biology, Physiology and Immunology, Avda Menéndez Pidal s/n 14004, Córdoba, Spain. Electronic address: e.munoz@uco.es.

Abstract

Black chokeberry has been known to play a protective role in human health due to its high polyphenolic content including anthocyanins and caffeic acid derivatives. In the present study, we first characterized the polyphenolic content of a commercial chokeberry concentrate and investigated its effect on LPS-induced NF-κB activation and release of pro-inflammatory mediators in macrophages in the presence or the absence of sodium selenite. Examination of the phytochemical profile of the juice concentrate revealed high content of polyphenols (3.3%), including anthocyanins, proanthocyanidins, phenolic acids, and flavonoids. Among them, cyanidin-3-O-galactoside and caffeoylquinic acids were identified as the major compounds. Data indicated that chokeberry concentrate inhibited both the release of TNFα, IL-6 and IL-8 in human peripheral monocytes and the activation of the NF-κB pathway in RAW 264.7 macrophage cells. Furthermore, chokeberry synergizes with sodium selenite to inhibit NF-κB activation, cytokine release and PGE2 synthesis. These findings suggest that selenium added to chokeberry juice enhances significantly its anti-inflammatory activity, thus revealing a sound approach in order to tune the use of traditional herbals by combining them with micronutrients.

KEYWORDS:

Aronia melanocarpa; Cytokines; NF-κB; Prostaglandin E(2); Selenium

PMID:
26079445
DOI:
10.1016/j.fitote.2015.06.009
[Indexed for MEDLINE]

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