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Evid Based Complement Alternat Med. 2015;2015:107814. doi: 10.1155/2015/107814. Epub 2015 May 20.

Triptolide Attenuates Podocyte Injury by Regulating Expression of miRNA-344b-3p and miRNA-30b-3p in Rats with Adriamycin-Induced Nephropathy.

Author information

1
Department of Graduate School, Nanjing University of Chinese Medicine, Nanjing 210023, China ; Department of Nephrology, Suzhou Hospital of Chinese Medicine, Suzhou 215000, China.
2
The First Affiliated Hospital of Soochow University, Suzhou 21500, China.
3
Department of Graduate School, Nanjing University of Chinese Medicine, Nanjing 210023, China.
4
Department of Nephrology, Wuxi No. 3 People's Hospital, Wuxi 214000, China.
5
Department of Nephrology, Suzhou Hospital of Chinese Medicine, Suzhou 215000, China.
6
Department of Nephrology, Jiangsu Provincial Hospital of Chinese Medicine, Affiliated Hospital of Nanjing University of Chinese Medicine, Nanjing 210029, China.

Abstract

OBJECTIVES:

We investigated the action of triptolide in rats with adriamycin-induced nephropathy and evaluated the possible mechanisms underlying its protective effect against podocyte injury.

METHODS:

In total, 30 healthy male Sprague-Dawley rats were randomized into three groups (normal group, model group, and triptolide group). On days 7, 28, 42, and 56, 24 h urine samples were collected. All rats were sacrificed on day 56, and their blood and renal tissues were collected for determination of biochemical and molecular biological parameters. Expression of miRNAs in the renal cortex was analyzed by a biochip assay and RT-PCR was used to confirm observed differences in miRNA levels.

RESULTS:

Triptolide decreased proteinuria, improved renal function without apparent adverse effects on the liver, and alleviated renal pathological lesions. Triptolide also elevated the nephrin protein level. Furthermore, levels of miR-344b-3p and miR-30b-3p were elevated in rats with adriamycin-induced nephropathy, while triptolide treatment reversed the increase in the expression of these two miRNAs.

CONCLUSIONS:

These results suggest that triptolide may attenuate podocyte injury in rats with adriamycin-induced nephropathy by regulating expression of miRNA-344b-3p and miRNA-30b-3p.

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