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Nat Commun. 2015 Jun 16;6:7394. doi: 10.1038/ncomms8394.

Polymerase Θ is a key driver of genome evolution and of CRISPR/Cas9-mediated mutagenesis.

Author information

1
Department of Human Genetics, Leiden University Medical Center, PO Box 9600, 2300 RC, Leiden, The Netherlands.
2
Department of Biology, Division of Developmental Biology, Utrecht University, Padualaan 8, 3584 CH, Utrecht, The Netherlands.

Abstract

Cells are protected from toxic DNA double-stranded breaks (DSBs) by a number of DNA repair mechanisms, including some that are intrinsically error prone, thus resulting in mutations. To what extent these mechanisms contribute to evolutionary diversification remains unknown. Here, we demonstrate that the A-family polymerase theta (POLQ) is a major driver of inheritable genomic alterations in Caenorhabditis elegans. Unlike somatic cells, which use non-homologous end joining (NHEJ) to repair DNA transposon-induced DSBs, germ cells use polymerase theta-mediated end joining, a conceptually simple repair mechanism requiring only one nucleotide as a template for repair. Also CRISPR/Cas9-induced genomic changes are exclusively generated through polymerase theta-mediated end joining, refuting a previously assumed requirement for NHEJ in their formation. Finally, through whole-genome sequencing of propagated populations, we show that only POLQ-proficient animals accumulate genomic scars that are abundantly present in genomes of wild C. elegans, pointing towards POLQ as a major driver of genome diversification.

PMID:
26077599
PMCID:
PMC4490562
DOI:
10.1038/ncomms8394
[Indexed for MEDLINE]
Free PMC Article

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