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BMB Rep. 2015 Dec;48(12):691-5.

Phytosphingosine promotes megakaryocytic differentiation of myeloid leukemia cells.

Author information

1
Departments of 1Life Science, Ewha Womans University, Seoul 03760, Korea.
2
Departments of Life Science, Ewha Womans University, Seoul 03760, Korea.
3
Bio-informatics Science, Ewha Womans University, Seoul 03760, Korea.
4
BK21 Plus R-FIND Team, College of Pharmacy, Dongguk University, Goyang 10326, Korea.
5
Doosan Corporation, Suwon 16229, Korea.

Abstract

We report that phytosphingosine, a sphingolipid found in many organisms and implicated in cellular signaling, promotes megakaryocytic differentiation of myeloid leukemia cells. Specifically, phytosphingosine induced several hallmark changes associated with megakaryopoiesis from K562 and HEL cells including cell cycle arrest, cell size increase and polyploidization. We also confirmed that cell type specific markers of megakaryocytes, CD41a and CD42b are induced by phytosphingosine. Phospholipids with highly similar structures were unable to induce similar changes, indicating that the activity of phytosphingosine is highly specific. Although phytosphingosine is known to activate p38 MAPK-mediated apoptosis, the signaling mechanisms involved in megakaryopoiesis appear to be distinct. In sum, we present another model for dissecting molecular details of megakaryocytic differentiation which in large part remains obscure.

PMID:
26077028
PMCID:
PMC4791325
[Indexed for MEDLINE]
Free PMC Article

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