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Stem Cells Dev. 2015 Oct 1;24(19):2307-16. doi: 10.1089/scd.2015.0031. Epub 2015 Aug 24.

Derivation of a Homozygous Human Androgenetic Embryonic Stem Cell Line.

Ding C1,2, Huang S1,2, Qi Q1,2, Fu R3, Zhu W3, Cai B1,2, Hong P1,2, Liu Z3, Gu T3, Zeng Y1,2, Wang J1,2, Xu Y1,2, Zhao X3, Zhou Q3, Zhou C1,2.

Author information

1
1 Reproductive Medicine Center, First Affiliated Hospital of Sun Yat-sen University , Guangdong, China .
2
2 The Key Laboratory of Reproductive Medicine of Guangdong Province , Guangdong, China .
3
3 State Key Laboratory of Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences , Beijing, China .

Abstract

Human embryonic stem cells (hESCs) have long been considered as a promising source for cell replacement therapy. However, one major obstacle for the use of these cells is immune compatibility. Histocompatible human parthenogenetic ESCs have been reported as a new method for generating human leukocyte antigen (HLA)-matched hESCs. To further investigate the possibility of obtaining histocompatible stem cells from uniparental embryos, we tried to produce androgenetic haploid human embryos by injecting a single spermatozoon into enucleated human oocyte, and establish human androgenetic embryonic stem (hAGES) cell lines from androgenetic embryos. In the present study, a diploid hAGES cell line has been established, which exhibits typical features of human ESCs, including the expression of pluripotency markers, having differentiation potential in vitro and in vivo, and stable propagation in an undifferentiated state (>P40). Bisulfite sequencing of the H19, Snrpn, Meg3, and Kv imprinting control regions suggested that hAGES cells maintained to a certain extent a sperm methylation pattern. Genome-wide single nucleotide polymorphism, short tandem repeat, and HLA analyses revealed that the hAGES cell genome was highly homozygous. These results suggest that hAGES cells from spermatozoon could serve as a useful tool for studying the mechanisms underlying genomic imprinting in humans. It might also be used as a potential resource for cell replacement therapy as parthenogenetic stem cells.

PMID:
26076706
DOI:
10.1089/scd.2015.0031
[Indexed for MEDLINE]

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