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Cell Rep. 2015 Jun 23;11(11):1703-13. doi: 10.1016/j.celrep.2015.05.030. Epub 2015 Jun 11.

Helicobacter pylori Infection Causes Characteristic DNA Damage Patterns in Human Cells.

Author information

1
Department of Molecular Biology, Max Planck Institute for Infection Biology, Charitéplatz 1, 10117 Berlin, Germany.
2
Department of Molecular Biology, Max Planck Institute for Infection Biology, Charitéplatz 1, 10117 Berlin, Germany. Electronic address: meyer@mpiib-berlin.mpg.de.

Abstract

Infection with the human pathogen Helicobacter pylori (H. pylori) is a major risk factor for gastric cancer. Since the bacterium exerts multiple genotoxic effects, we examined the circumstances of DNA damage accumulation and identified regions within the host genome with high susceptibility to H. pylori-induced damage. Infection impaired several DNA repair factors, the extent of which depends on a functional cagPAI. This leads to accumulation of a unique DNA damage pattern, preferentially in transcribed regions and proximal to telomeres, in both gastric cell lines and primary gastric epithelial cells. The observed pattern correlates with focal amplifications in adenocarcinomas of the stomach and partly overlaps with known cancer genes. We thus demonstrate an impact of a bacterial infection directed toward specific host genomic regions and describe underlying characteristics that make such regions more likely to acquire heritable changes during infection, which could contribute to cellular transformation.

PMID:
26074077
DOI:
10.1016/j.celrep.2015.05.030
[Indexed for MEDLINE]
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