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Diagn Microbiol Infect Dis. 2015 Sep;83(1):18-20. doi: 10.1016/j.diagmicrobio.2015.05.011. Epub 2015 May 27.

Detection of enteropathogens associated with travelers' diarrhea using a multiplex Luminex-based assay performed on stool samples smeared on Whatman FTA Elute cards.

Author information

  • 1Infectious Disease Clinic, Naval Medical Center, Portsmouth, VA, USA; Infectious Disease Clinical Research Program, Uniformed Services University, Bethesda, MD, USA; Henry M Jackson Foundation for the Advancement of Military Medicine, Bethesda, MD, USA. Electronic address: tlalani@idcrp.org.
  • 2Infectious Disease Clinic, Naval Medical Center, Portsmouth, VA, USA; Infectious Disease Clinical Research Program, Uniformed Services University, Bethesda, MD, USA; Henry M Jackson Foundation for the Advancement of Military Medicine, Bethesda, MD, USA.
  • 3Infectious Disease Clinic, Naval Medical Center, Portsmouth, VA, USA.
  • 4Independent Consultant, San Angelo, TX, USA.
  • 5Enteric Diseases Department, Naval Medical Research Center, Silver Spring, MD, USA.
  • 6Infectious Disease Clinical Research Program, Uniformed Services University, Bethesda, MD, USA.

Abstract

We evaluated the limits of detection (LoD) for an 11-plex PCR-Luminex assay performed on Whatman(™) FTA Elute cards smeared with stool containing pathogens associated with travelers' diarrhea. LoDs ranged from 10(2) to 10(5)CFU, PFU, or cysts/g for most pathogens except Cryptosporidium. Campylobacter and norovirus LoDs increased with prolonged storage of cards.

KEYWORDS:

PCR; Stool card; Travelers' diarrhea

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