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J Chromatogr Sci. 2015 Nov-Dec;53(10):1685-94. doi: 10.1093/chromsci/bmv072. Epub 2015 Jun 12.

Quality control of Gardeniae Fructus by HPLC-PDA fingerprint coupled with chemometric methods.

Author information

1
College of Pharmacy, Nanjing University of Chinese Medicine, Nanjing 210023, China.
2
Department of Chinese Medicine, Jiangsu Cancer Hospital, Nanjing 210009, China.
3
College of Pharmacy, Nanjing University of Chinese Medicine, Nanjing 210023, China The State Key Lab of Pharmaceutical Biotechnology, College of Life Sciences, Nanjing University, Nanjing 210093, China Jiangsu Key Laboratory of Pediatric Respiratory Disease, Nanjing University of Chinese Medicine, Nanjing 210023, China wyin@nju.edu.cn.

Abstract

The ripe fruits of Gardenia jasminoides Ellis have been used as traditional Chinese medicine to treat diseases for a long history. Lines of evidence demonstrate that multiple active constituents are responsible for the therapeutic effects of this herbal medicine. However, effective methods for quality control of this herbal medicine are still lacking. In this study, a high-performance liquid chromatography (HPLC) fingerprint analysis was performed on a SinoChrom ODS-BP C18 column (4.6 mm × 250 mm, 5 μm) at 30°C with mobile phase of aqueous solution with 0.1% formic acid and acetonitrile. On the basis of the chromatographic data from 32 batches samples, the HPLC fingerprint pattern containing 27 common peaks was obtained. Among these common peaks, seven peaks were identified by the electrospray ionization-mass spectrometry as geniposidic acid, genipin-1-β-gentiobioside, chlorogenic acid, geniposide, rutin, crocin-1 and crocin-2 and the contents of these seven compounds were simultaneously determined. Finally, chemometric methods including hierarchical clustering analysis and principal component analysis were successfully applied to differentiate the samples from six producing regions. In sum, the data, as described in this study, offer valuable information for the quality control and proper use of Gardeniae Fructus.

PMID:
26071608
DOI:
10.1093/chromsci/bmv072
[Indexed for MEDLINE]

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