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Science. 2015 Jun 12;348(6240):1217. doi: 10.1126/science.aaa8299. Epub 2015 Jun 11.

LOCAL TRANSLATION. Response to Comment on "Principles of ER cotranslational translocation revealed by proximity-specific ribosome profiling".

Author information

1
Department of Cellular and Molecular Pharmacology, Howard Hughes Medical Institute, California Institute for Quantitative Biosciences, Center for RNA Systems Biology, University of California-San Francisco, San Francisco, CA 94158, USA.
2
Department of Cellular and Molecular Pharmacology, Howard Hughes Medical Institute, California Institute for Quantitative Biosciences, Center for RNA Systems Biology, University of California-San Francisco, San Francisco, CA 94158, USA. jonathan.weissman@ucsf.edu.

Abstract

Reid and Nicchitta propose that most cellular translation is carried out by a noncycling pool of endoplasmic reticulum (ER)-associated ribosomes. However, proximity-specific ribosome profiling data place an upper bound of about 7 to 16% on the fraction of cytosolic protein translation carried out by ribosomes accessible to ER-tethered biotin ligases. Moreover, yeast pulse-labeling experiments argue against there being a static population of ER-associated ribosomes.

PMID:
26068842
DOI:
10.1126/science.aaa8299
[Indexed for MEDLINE]
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