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Clin Transl Oncol. 2015 Oct;17(10):779-87. doi: 10.1007/s12094-015-1303-1. Epub 2015 Jun 11.

Expression of serum miR-200a, miR-200b, and miR-200c as candidate biomarkers in epithelial ovarian cancer and their association with clinicopathological features.

Author information

1
Department of Biochemistry, Maulana Azad Medical College, New Delhi, 110002, India.
2
Prince Fahd Bin Sultan Research Chair, University of Tabuk, Tabuk, 71491, Saudi Arabia.
3
Department of Biochemistry, Maulana Azad Medical College, New Delhi, 110002, India. alpanasaxena@hotmail.com.

Abstract

BACKGROUND:

MicroRNAs (miRs) have been implicated in the etiology of various human cancers. The aim of this study was to investigate the association of the expression of three members--miR 200a, miR 200b, and miR 200c belonging to the miR-200 family with clinicopathological characteristics and their impact on the progression of epithelial ovarian cancer (EOC).

MATERIALS AND METHODS:

Total RNA from serum was isolated by Trizol method, polyadenylated, and reverse transcribed into cDNA. Expression levels of miR-200a, miR-200b, and miR-200c were detected by using miRNA qRT-PCR. We measured miR expression in 70 serum samples of EOC patients with matched controls using U6 snRNA as a reference. Levels of miR expression was compared with distinct clinicopathological features.

RESULTS:

Expression of miR-200a was found to be greater than six-fold (p = 0.01), miR-200b and miR-200c greater than three-fold (p = 0.01) in comparison with matched normal controls. Association of miRNA expression with clinicopathological factors and progression was statistically evaluated. The expression levels of miR-200a and miR-200c were found to be significantly associated with disease progression (p = 0.04 and p < 0.001, respectively). miR-200a overexpression was found be associated with tumor histology and stage. Patients with lymph node metastasis showed significant elevation of miR-200c (p = 0.006). The AUC in ROC curve also indicated that serum levels of miR-200a and miR-200c might be worthwhile as a diagnostic tool in the near future.

CONCLUSION:

Our findings suggest that miR-200a, miR-200b, and miR-200c overexpressions are associated with the aggressive tumor progression and be recognized as reliable markers to predict the prognosis and survival in EOC patients.

KEYWORDS:

Biomarker; Disease progression; Overexpression; qRT-PCR

PMID:
26063644
DOI:
10.1007/s12094-015-1303-1
[Indexed for MEDLINE]

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