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Acta Biochim Biophys Sin (Shanghai). 2015 Jul;47(7):504-15. doi: 10.1093/abbs/gmv045. Epub 2015 Jun 10.

Alteration of sperm protein profile induced by cigarette smoking.

Author information

1
School of Life Sciences and Biotechnology, Shanghai Key Laboratory of Reproductive Medicine, Shanghai Jiao Tong University, Shanghai 200240, China Shanghai Institute of Medical Genetics, Shanghai 200040, China.
2
School of Life Sciences and Biotechnology, Shanghai Key Laboratory of Reproductive Medicine, Shanghai Jiao Tong University, Shanghai 200240, China.
3
China National Population and Family Planning Key Laboratory of Contraceptive Drugs and Devices, SIPPR, Fudan University, Shanghai 200032, China.
4
Department of Urology, Shanghai Jiao Tong University Affiliated Sixth People's Hospital, Shanghai 200233, China zdqiao@sjtu.edu.cn chenzhongchina@hotmail.com.
5
School of Life Sciences and Biotechnology, Shanghai Key Laboratory of Reproductive Medicine, Shanghai Jiao Tong University, Shanghai 200240, China Shanghai Institute of Medical Genetics, Shanghai 200040, China zdqiao@sjtu.edu.cn chenzhongchina@hotmail.com.

Abstract

Cigarette smoking is associated with lower semen quality, but how cigarette smoking changes the semen quality remains unclear. The aim of this study was to screen the differentially expressed proteins in the sperm of mice with daily exposure to cigarette smoke. The 2D gel electrophoresis (2DE) and mass spectrometry (MS) analyses results showed that the mouse sperm protein profile was altered by cigarette smoking. And 22 of the most abundant proteins that correspond to differentially expressed spots in 2DE gels of the sperm samples were identified. These proteins were classified into different groups based on their functions, such as energy metabolism, reproduction, and structural molecules. Furthermore, the 2DE and MS results of five proteins (Aldoa, ATP5a1, Gpx4, Cs, and Spatc1) were validated by western blot analysis and reverse transcriptase-polymerase chain reaction. Results showed that except Spatc1 the other four proteins showed statistically significant different protein levels between the smoking group and the control group (P < 0.05). The expressions of three genes (Aldoa, Gpx4, and Spatc1) were significantly different (P < 0.05) at transcription level between the smoking group and the control group. In addition, five proteins (Aldoa, ATP5a1, Spatc1, Cs, and Gpx4) in human sperm samples from 30 male smokers and 30 non-smokers were detected by western blot analysis. Two proteins (Aldoa and Cs) that are associated with energy production were found to be significantly altered, suggesting that these proteins may be potential diagnostic markers for evaluation of smoking risk in sperm. Further study of these proteins may provide insight into the pathogenic mechanisms underlying infertility in smoking persons.

KEYWORDS:

cigarette smoking; male fertility; mass spectrometry; protein profile; sperm

PMID:
26063603
DOI:
10.1093/abbs/gmv045
[Indexed for MEDLINE]

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