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ACS Chem Biol. 2015 Sep 18;10(9):1958-63. doi: 10.1021/acschembio.5b00256. Epub 2015 Jun 22.

A Fluorescent Sensor and Gel Stain for Detection of Pyrophosphorylated Proteins.

Author information

1
Department of Chemistry, Princeton University , Washington Road, Princeton, New Jersey 08544, United States.

Abstract

The design and synthesis of a fluorescent sensor of diphosphate esters along with its application for in-gel detection is described. Dinuclear zinc complex 1 selectively binds diphosphate esters in the presence of various other functional groups, including monophosphate esters. Complex 1 also constitutes a competent stain for visualization of pyrophosphorylated proteins in polyacrylamide gels. This reagent will facilitate the validation and exploration of protein pyrophosphorylation.

PMID:
26061479
DOI:
10.1021/acschembio.5b00256
[Indexed for MEDLINE]

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