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Methods Mol Biol. 2015;1319:233-43. doi: 10.1007/978-1-4939-2748-7_13.

Electrochemical Glucose Biosensor Based on Glucose Oxidase Displayed on Yeast Surface.

Author information

1
Laboratory for Biosensing, Qingdao Institute of Bioenergy & Bioprocess Technology (QIBEBT) and Key Laboratory of Biofuels (QIBEBT), Chinese Academy of Sciences, 189 Songling Road, Qingdao, 266101, China.

Abstract

The conventional enzyme-based biosensor requires chemical or physical immobilization of purified enzymes on electrode surface, which often results in loss of enzyme activity and/or fractions immobilized over time. It is also costly. A major advantage of yeast surface display is that it enables the direct utilization of whole cell catalysts with eukaryote-produced proteins being displayed on the cell surface, providing an economic alternative to traditional production of purified enzymes. Herein, we describe the details of the display of glucose oxidase (GOx) on yeast cell surface and its application in the development of electrochemical glucose sensor. In order to achieve a direct electrochemistry of GOx, the entire cell catalyst (yeast-GOx) was immobilized together with multiwalled carbon nanotubes on the electrode, which allowed sensitive and selective glucose detection.

PMID:
26060079
DOI:
10.1007/978-1-4939-2748-7_13
[Indexed for MEDLINE]

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