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Int J Urol. 2015 Sep;22(9):867-77. doi: 10.1111/iju.12829. Epub 2015 Jun 7.

MicroRNA-205 inhibits cancer cell migration and invasion via modulation of centromere protein F regulating pathways in prostate cancer.

Author information

1
Department of Functional Genomics, Chiba University Graduate School of Medicine, Chiba, Japan.
2
Department of Urology, Chiba University Graduate School of Medicine, Chiba, Japan.
3
Department of Urology, Graduate School of Medical and Dental Sciences, Kagoshima University, Kagoshima, Japan.
4
Department of Urology, Teikyo University Chiba Medical Center, Chiba, Japan.

Abstract

OBJECTIVES:

To investigate the functional roles of microRNA-205 in the modulation of novel cancer pathways in prostate cancer cells.

METHODS:

Functional studies of microRNA-205 were carried out to investigate cell proliferation, migration and invasion in prostate cancer cell lines (PC3 and DU145) by restoration of mature microRNA. In silico database and genome-wide gene expression analyses were carried out to identify molecular targets and pathways mediated by microRNA-205. Loss-of-function studies were applied to microRNA-205 target genes.

RESULTS:

Restoration of microRNA-205 in cancer cell lines significantly inhibited cancer cell migration and invasion. Our data showed that the centromere protein F gene was overexpressed in prostate cancer clinical specimens and was a direct target of microRNA-205 regulation. Silencing of centromere protein F significantly inhibited cancer cell migration and invasion. Furthermore, MCM7, an oncogenic gene functioning downstream of centromere protein F, was identified by si-centromere protein F transfectants in prostate cancer cells.

CONCLUSIONS:

Loss of tumor-suppressive microRNA-205 seems to enhance cancer cell migration and invasion in prostate cancer through direct regulation of centromere protein F. Our data describing pathways regulated by tumor-suppressive microRNA-205 provide new insights into the potential mechanisms of prostate cancer oncogenesis and metastasis.

KEYWORDS:

centromere protein F; microRNA; microRNA-205; prostate cancer; tumor suppressor

PMID:
26059417
DOI:
10.1111/iju.12829
[Indexed for MEDLINE]
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