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PLoS One. 2015 Jun 9;10(6):e0129273. doi: 10.1371/journal.pone.0129273. eCollection 2015.

Membrane-Associated Transporter Protein (MATP) Regulates Melanosomal pH and Influences Tyrosinase Activity.

Author information

1
Bioscience Research Institute, Amorepacific Corporation R&D Center, Yongin, Republic of Korea.
2
Department of Chemical Engineering, POSTECH, Pohang, Republic of Korea.
3
Gyeonggi Bio Center, Gyeonggi Institute of Science & Technology Promotion, Suwon, Republic of Korea.
4
Department of Dermatology, Dongguk University Ilsan Hospital, Goyang, Republic of Korea.

Abstract

The SLC45A2 gene encodes a Membrane-Associated Transporter Protein (MATP). Mutations of this gene cause oculocutaneous albinism type 4 (OCA4). However, the molecular mechanism of its action in melanogenesis has not been elucidated. Here, we discuss the role of MATP in melanin production. The SLC45A2 gene is highly enriched in human melanocytes and melanoma cell lines, and its protein, MATP, is located in melanosomes. The knockdown of MATP using siRNAs reduced melanin content and tyrosinase activity without any morphological change in melanosomes or the expression of melanogenesis-related proteins. Interestingly, the knockdown of MATP significantly lowered the melanosomal pH, as verified through DAMP analysis, suggesting that MATP regulates melanosomal pH and therefore affects tyrosinase activity. Finally, we found that the reduction of tyrosinase activity associated with the knockdown of MATP was readily recovered by copper treatment in the in vitro L-DOPA oxidase activity assay of tyrosinase. Considering that copper is an important element for tyrosinase activity and that its binding to tyrosinase depends on melanosomal pH, MATP may play an important role in regulating tyrosinase activity via controlling melanosomal pH.

PMID:
26057890
PMCID:
PMC4461305
DOI:
10.1371/journal.pone.0129273
[Indexed for MEDLINE]
Free PMC Article

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