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Environ Toxicol Pharmacol. 2015 Jul;40(1):30-8. doi: 10.1016/j.etap.2015.05.006. Epub 2015 May 16.

Anti-metastatic effects of Rheum Palmatum L. extract in human MDA-MB-231 breast cancer cells.

Author information

1
Herbal Medicine Resources Group, Korea Institute of Oriental Medicine, 1672 Yuseong-daero, Yuseong-gu, Daejeon 305-811, Republic of Korea.
2
Herbal Medicine Resources Group, Korea Institute of Oriental Medicine, 1672 Yuseong-daero, Yuseong-gu, Daejeon 305-811, Republic of Korea. Electronic address: hkkim@kiom.re.kr.

Abstract

Rheum palmatum L. (RP) has been widely used in traditional medicine for the treatment of various diseases in Asian countries. The molecular mechanism of its anti-metastasis effect remains elusive. The present study assessed the effect of RP ethanol extract (RPE) on the highly metastatic human MDA-MB-231 breast cancer cells in vitro. At a non-toxic concentration, RPE inhibited migration, motility and invasion in a concentration-dependent manner. To investigate the mechanisms involved, real-time PCR and Western blot analyses were performed. Results showed that RPE down-regulated the levels of extracellular matrix degradation-associated proteins, including MMP-2/-9, uPA and uPAR, and up-regulated PAI-1. In addition, RPE affected NF-κB by degrading IkBα, and affected the mitogen-activated protein kinase signal transduction pathway by depressing the activation of p38, ERK and Akt. These results suggest that RPE has potential anti-metastatic activity and warrants further investigation.

KEYWORDS:

Invasion, Matrix metalloproteinases; MDA-MB-231; Nuclear factor-κB; Rheum palmatum L.; Urokinase-type plasminogen activator

PMID:
26056975
DOI:
10.1016/j.etap.2015.05.006
[Indexed for MEDLINE]

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