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J Cell Biol. 2015 Jun 8;209(5):633-44. doi: 10.1083/jcb.201409074.

Nanobodies and recombinant binders in cell biology.

Author information

1
Department of Biology II, Ludwig Maximilians University Munich and Center for Integrated Protein Science Munich, 82152 Planegg-Martinsried, Germany.
2
Department of Biology, Technical University of Darmstadt, 64287 Darmstadt, Germany.
3
Laboratory of Cellular and Molecular Immunology, Vrije Universiteit Brussel, 1050 Brussels, Belgium.
4
Department of Biology II, Ludwig Maximilians University Munich and Center for Integrated Protein Science Munich, 82152 Planegg-Martinsried, Germany h.leonhardt@lmu.de.

Abstract

Antibodies are key reagents to investigate cellular processes. The development of recombinant antibodies and binders derived from natural protein scaffolds has expanded traditional applications, such as immunofluorescence, binding arrays, and immunoprecipitation. In addition, their small size and high stability in ectopic environments have enabled their use in all areas of cell research, including structural biology, advanced microscopy, and intracellular expression. Understanding these novel reagents as genetic modules that can be integrated into cellular pathways opens up a broad experimental spectrum to monitor and manipulate cellular processes.

PMID:
26056137
PMCID:
PMC4460151
DOI:
10.1083/jcb.201409074
[Indexed for MEDLINE]
Free PMC Article

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