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Biochem Biophys Res Commun. 2015 Aug 7;463(4):859-63. doi: 10.1016/j.bbrc.2015.06.025. Epub 2015 Jun 6.

Involvement of trefoil factor family 2 in the enlargement of intestinal tumors in Apc(Min/+) mice.

Author information

1
Division of Molecular Biology, Department of Pharmacy, Nagasaki International University, 2825-7 Huis Ten Bosch, Sasebo, Nagasaki 859 3298, Japan. Electronic address: kfujit@niu.ac.jp.
2
Division of Cancer Prevention Research, National Cancer Center Research Institute, 5-1-1 Tsukiji, Cyuo-ku, Tokyo 104 0045, Japan. Electronic address: gfujii@ncc.go.jp.
3
Department of Pathology, National Kyushu Cancer Center, 3-1-1 Notame, Minami-ku, Fukuoka 811 1395, Japan. Electronic address: kentag@nk-cc.go.jp.
4
Cell Innovator Inc., 806, Westwing, 3-1-1 Maidashi, Higashi-ku, Fukuoka 812 8582, Japan. Electronic address: kaori_yasuda@cell-innovator.com.
5
Division of Molecular Biology, Department of Pharmacy, Nagasaki International University, 2825-7 Huis Ten Bosch, Sasebo, Nagasaki 859 3298, Japan. Electronic address: cruise-sinlove@docomo.ne.jp.
6
Division of Molecular Biology, Department of Pharmacy, Nagasaki International University, 2825-7 Huis Ten Bosch, Sasebo, Nagasaki 859 3298, Japan. Electronic address: k-bb.4ev@ezweb.ne.jp.
7
Division of Cancer Prevention Research, National Cancer Center Research Institute, 5-1-1 Tsukiji, Cyuo-ku, Tokyo 104 0045, Japan; Epidemiology and Prevention Division, Research Centre for Cancer Prevention and Screening, National Cancer Center, 5-1-1 Tsukiji, Cyuo-ku, Tokyo 104 0045, Japan. Electronic address: mimutoh@ncc.go.jp.
8
Division of Molecular Biology, Department of Pharmacy, Nagasaki International University, 2825-7 Huis Ten Bosch, Sasebo, Nagasaki 859 3298, Japan. Electronic address: h-tanaka@niu.ac.jp.
9
Division of Molecular Biology, Department of Pharmacy, Nagasaki International University, 2825-7 Huis Ten Bosch, Sasebo, Nagasaki 859 3298, Japan. Electronic address: wadam@niu.ac.jp.

Abstract

It is assumed that tumor size may be associated with malignant tumor conversion. However, the molecules responsible for determination of tumor size are not well understood. We counted the number of intestinal tumors in 8, 12 and 30-week-old Apc(Min/+) mice and measured tumor sizes, respectively. Genes involved in determining tumor size were examined using microarray analysis. Cultured cells were then, transfected with a mammalian expression vector containing a candidate gene to examine the functional role of the gene. The effect of forced expression of candidate gene on cell growth was evaluated by measuring the doubling time of the cultured cells and the growth of grafted cells in nude mice. Unexpectedly, microarray analysis identified trefoil factor family 2 (Tff2) rather than growth related genes and/or oncogenes as a most variable gene. Overexpressing Tff2 in cultured cells reduced doubling time in vitro and rapidly increased xenograft tumor size in vivo. We found Tff2 as a novel important factor that to be able to enlarge an intestinal tumor size.

KEYWORDS:

Apc(Min/+) mice; Microarray; Proliferation; Trefoil factor family 2; Tumor size

PMID:
26056002
DOI:
10.1016/j.bbrc.2015.06.025
[Indexed for MEDLINE]
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