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FEMS Yeast Res. 2015 Aug;15(5):fov038. doi: 10.1093/femsyr/fov038. Epub 2015 Jun 7.

Coaggregation of Candida albicans, Actinomyces naeslundii and Streptococcus mutans is Candida albicans strain dependent.

Author information

1
Oral Health CRC, Melbourne Dental School, The University of Melbourne, VIC 3053, Australia Kulliyyah of Dentistry, International Islamic University Malaysia, 25200 Kuantan, Pahang, Malaysia.
2
Oral Health CRC, Melbourne Dental School, The University of Melbourne, VIC 3053, Australia.
3
Oral Health CRC, Melbourne Dental School, The University of Melbourne, VIC 3053, Australia m.mccullough@unimelb.edu.au.

Abstract

Microbial interactions are necessarily associated with the development of polymicrobial oral biofilms. The objective of this study was to determine the coaggregation of eight strains of Candida albicans with Actinomyces naeslundii and Streptococcus mutans. In autoaggregation assays, C. albicans strains were grown in RPMI-1640 and artificial saliva medium (ASM) whereas bacteria were grown in heart infusion broth. C. albicans, A. naeslundii and S. mutans were suspended to give 10(6), 10(7) and 10(8) cells mL(-1) respectively, in coaggregation buffer followed by a 1 h incubation. The absorbance difference at 620 nm (ΔAbs) between 0 h and 1 h was recorded. To study coaggregation, the same protocol was used, except combinations of microorganisms were incubated together. The mean ΔAbs% of autoaggregation of the majority of RPMI-1640-grown C. albicans was higher than in ASM grown. Coaggregation of C. albicans with A. naeslundii and/or S. mutans was variable among C. albicans strains. Scanning electron microscopy images showed that A. naeslundii and S. mutans coaggregated with C. albicans in dual- and triculture. In conclusion, the coaggregation of C. albicans, A. naeslundii and S. mutans is C. albicans strain dependent.

KEYWORDS:

aggregation; hyphal form; yeast form

PMID:
26054855
DOI:
10.1093/femsyr/fov038
[Indexed for MEDLINE]

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