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Nat Immunol. 2015 Jul;16(7):718-28. doi: 10.1038/ni.3200. Epub 2015 Jun 8.

Identification of cDC1- and cDC2-committed DC progenitors reveals early lineage priming at the common DC progenitor stage in the bone marrow.

Author information

1
Singapore Immunology Network, Agency for Science, Technology and Research, Singapore.
2
Genome Institute of Singapore, Agency for Science, Technology and Research, Singapore.
3
Molecular Medicine Division, Walter and Eliza Hall Institute of Medical Research, Parkville, Australia.
4
1] Singapore Immunology Network, Agency for Science, Technology and Research, Singapore. [2] Department of Microbiology, Yong Loo Lin School of Medicine, National University of Singapore, National University Health System, Singapore.
5
1] Molecular Medicine Division, Walter and Eliza Hall Institute of Medical Research, Parkville, Australia. [2] Department of Medical Biology, University of Melbourne, Parkville, Australia.

Abstract

Mouse conventional dendritic cells (cDCs) can be classified into two functionally distinct lineages: the CD8α(+) (CD103(+)) cDC1 lineage, and the CD11b(+) cDC2 lineage. cDCs arise from a cascade of bone marrow (BM) DC-committed progenitor cells that include the common DC progenitors (CDPs) and pre-DCs, which exit the BM and seed peripheral tissues before differentiating locally into mature cDCs. Where and when commitment to the cDC1 or cDC2 lineage occurs remains poorly understood. Here we found that transcriptional signatures of the cDC1 and cDC2 lineages became evident at the single-cell level from the CDP stage. We also identified Siglec-H and Ly6C as lineage markers that distinguished pre-DC subpopulations committed to the cDC1 lineage (Siglec-H(-)Ly6C(-) pre-DCs) or cDC2 lineage (Siglec-H(-)Ly6C(+) pre-DCs). Our results indicate that commitment to the cDC1 or cDC2 lineage occurs in the BM and not in the periphery.

PMID:
26054720
DOI:
10.1038/ni.3200
[Indexed for MEDLINE]

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