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Fertil Steril. 2015 Sep;104(3):561-8.e4. doi: 10.1016/j.fertnstert.2015.05.018. Epub 2015 Jun 18.

Total levels, localization patterns, and proportions of sperm exhibiting phospholipase C zeta are significantly correlated with fertilization rates after intracytoplasmic sperm injection.

Author information

1
Nuffield Department of Obstetrics and Gynaecology, University of Oxford, Women's Centre, John Radcliffe Hospital, Headington, Oxford, United Kingdom.
2
Medical Research Institute, University of Dundee, Ninewells Hospital and Medical School, Dundee, United Kingdom; Assisted Conception Unit, National Health Service Tayside, Ninewells Hospital, Dundee, United Kingdom.
3
Nuffield Department of Obstetrics and Gynaecology, University of Oxford, Women's Centre, John Radcliffe Hospital, Headington, Oxford, United Kingdom. Electronic address: kevin.coward@obs-gyn.ox.ac.uk.

Abstract

OBJECTIVE:

To study the relationship of total levels, localization patterns, and proportions of sperm exhibiting phospholipase C zeta, with fertilization rates after in vitro fertilization (IVF) and intracytoplasmic sperm injection (ICSI).

DESIGN:

Laboratory study; controls vs. patients after IVF (n = 27) or ICSI (n = 17) treatment.

SETTING:

Fertility center.

PATIENT(S):

A total of 44 semen samples, subjected to either IVF or ICSI treatment. Oocyte collection, ICSI or IVF, determination of sperm concentration and motility, and immunocytochemical analyses of phospholipase C zeta (PLCζ).

INTERVENTION(S):

None.

MAIN OUTCOME MEASURE(S):

Percentages of sperm exhibiting PLCζ.

RESULT(S):

Significant positive correlation between ICSI fertilization rates and total levels, localization patterns, and the proportion (percentage) of sperm exhibiting PLCζ. Total levels, localization patterns, and the proportion of sperm exhibiting PLCζ are correlated with fertilization rates for ICSI, but not for IVF.

CONCLUSION(S):

Evaluating total levels, localization patterns, and proportions of PLCζ may represent a useful diagnostic tool for clinical purposes in men for whom IVF is not advised or has previously failed. This clinical study further supports the fundamental role of PLCζ in the oocyte activation process.

KEYWORDS:

In vitro fertilization; intracytoplasmic sperm injection; oocyte activation; phospholipase C zeta; total fertilization failure

[Indexed for MEDLINE]

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