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ACS Appl Mater Interfaces. 2015 Jul 1;7(25):13925-33. doi: 10.1021/acsami.5b02708. Epub 2015 Jun 19.

All-Aqueous Electrosprayed Emulsion for Templated Fabrication of Cytocompatible Microcapsules.

Song Y1,2, Chan YK2,3, Ma Q1,2, Liu Z1,2, Shum HC1,2.

Author information

1
‡HKU-Shenzhen Institute of Research and Innovation (HKU-SIRI), Guangdong 518000, China.
2
†Department of Mechanical Engineering, University of Hong Kong, Hong Kong.
3
§Department of Ophthalmology, University of Hong Kong, Hong Kong.

Abstract

Encapsulation of biomolecules and cells in hydrogel capsules via emulsion templating frequently induces an irreversible loss of bioactivity, because of the use of nonaqueous solvents. Here, we introduce an all-aqueous electrospray (AAE) approach to generate aqueous two-phase emulsion droplets, and we use them as templates to fabricate microcapsules with preserved cell viability. The approach allows formation of monodisperse microparticles with tunable sizes, variable compositions, and interior architectures in a mild gelation process. This technique potentially benefits a variety of new biomedical applications, such as delivery of bioactive proteins, transplantation of living cells, and assembly of cell-mimicking structures.

KEYWORDS:

all-aqueous droplets; biomolecular assembly; cell encapsulation; core−shell microcapsules; electrospray; w/w/w double emulsion

PMID:
26053733
DOI:
10.1021/acsami.5b02708
[Indexed for MEDLINE]

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