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J Recept Signal Transduct Res. 2015;35(6):605-12. doi: 10.3109/10799893.2015.1034368. Epub 2015 Jun 8.

Effects of dihydroxy gymnemic triacetate (DGT) on expression of apoptosis associated proteins in human prostate cancer cell lines (PC3).

Author information

1
a PG & Research Department of Biotechnology & Bioinformatics , Holy Cross College (Autonomous) , Tiruchirapalli , Tamil Nadu , India and.
2
b Department of Pharmaceutical Chemistry , Ultra College of Pharmacy , Madurai , Tamil Nadu , India.

Abstract

Prostate cancer is the most common malignancies among men. The present study is aimed at the investigation of dihydroxy gymnemic triacetate (DGT) from Gymnema sylvestre on mitochondrial apoptotic pathway and cell cycle arrest. Treatment of DGT resulted in a dose-dependent inhibition of growth of PC-3 cells. The cell cycle arrest was observed at the G2/M phase and accumulation of apoptotic cells was observed in DGT-treated prostate cancer cell lines. The occurrence of apoptosis in these cells was observed by DNA fragmentation. These events were associated with increased levels of pro-apoptotic proteins Bax, Bad and reduced levels of the antiapoptotic proteins Bcl-2, Bcl-xL and Mcl-1. DGT also induces the activation of caspase-9 and caspase-3. The above results, clearly, suggest that DGT induces apoptosis by the intrinsic pathways which could be very useful for the treatment of prostate cancer.

KEYWORDS:

Dihydroxy gymnemic triacetate; Gymnema sylvestre; PC-3; intrinsic; prostate cancer

PMID:
26053511
DOI:
10.3109/10799893.2015.1034368
[Indexed for MEDLINE]

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