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Trends Cell Biol. 2015 Aug;25(8):468-75. doi: 10.1016/j.tcb.2015.05.005. Epub 2015 Jun 4.

Single-molecule insights into mRNA dynamics in neurons.

Author information

1
Department of Anatomy and Structural Biology, Albert Einstein College of Medicine, 1300 Morris Park Avenue, Bronx, NY 10461, USA; Gruss Lipper Biophotonics Center, Albert Einstein College of Medicine, 1300 Morris Park Avenue, Bronx, NY 10461, USA; Janelia Farm Research Campus, Howard Hughes Medical Institute, 19700 Helix Drive, Ashburn, VA 20147, USA.
2
Department of Anatomy and Structural Biology, Albert Einstein College of Medicine, 1300 Morris Park Avenue, Bronx, NY 10461, USA.
3
Janelia Farm Research Campus, Howard Hughes Medical Institute, 19700 Helix Drive, Ashburn, VA 20147, USA; Department of Physics and Astronomy, Seoul National University, Seoul 151-747, Korea; Institute of Molecular Biology and Genetics, Seoul National University, Seoul 151-742, Korea. Electronic address: hyeyoon.park@snu.ac.kr.

Abstract

Targeting of mRNAs to neuronal dendrites and axons plays an integral role in intracellular signaling, development, and synaptic plasticity. Single-molecule imaging of mRNAs in neurons and brain tissue has led to enhanced understanding of mRNA dynamics. Here we discuss aspects of mRNA regulation as revealed by single-molecule detection, which has led to quantitative analyses of mRNA diversity, localization, transport, and translation. These exciting new discoveries propel our understanding of the life of an mRNA in a neuron and how its activity is regulated at the single-molecule level.

KEYWORDS:

RNA localization; local translation; mRNA tracking; smFISH

PMID:
26052005
PMCID:
PMC4515374
DOI:
10.1016/j.tcb.2015.05.005
[Indexed for MEDLINE]
Free PMC Article

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