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Immunol Lett. 2015 Aug;166(2):134-9. doi: 10.1016/j.imlet.2015.05.021. Epub 2015 Jun 5.

Investigation of CTLA-4-318C/T gene polymorphism in cases with type 1 diabetes of Azerbaijan, Northwest Iran.

Author information

1
Immunology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran; Department of Immunology, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran. Electronic address: almasysh@sums.ac.ir.
2
Drug Applied Research Center, Tabriz University of Medical Sciences, Tabriz, Iran; Department of Immunology, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran. Electronic address: aliparasty@sums.ac.ir.
3
Endocrine Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.
4
Department of Immunology, Faculty of Medicine, Qazvin University of Medical Sciences, Qazvin, Iran.
5
Department of Virology, School of Medicine, University of Tampere, Tampere, Finland; Fimlab laboratories, Pirkanmaa Hospital District, Tampere, Finland.
6
Children's Hospital, Helsinki University Central Hospital, University of Helsinki, Helsinki, Finland.
7
Department of Physiology, Faculty of Medicine, Zanjan University of Medical Sciences, Zanjan, Iran.
8
Department of Virology, School of Medicine, University of Tampere, Tampere, Finland; Department of Pathobiology, Faculty of Veterinary Medicine, University of Tabriz, Tabriz, Iran.
9
Department of Immunology, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran.

Abstract

This study analyzed the association of CTLA-4-318C/T gene polymorphism with susceptibility, clinical course and laboratory findings of Type 1 diabetes (T1D). One hundred and fifty-three T1D patients and 189 healthy controls entered this study. CTLA-4-318C/T genotyping was performed by tetra-primer amplification refractory mutation system-polymerase chain reaction (T-ARMS-PCR) analysis. The allelic and genotypic frequencies of -318C/T gene polymorphism were similar in patients and controls. However, younger age, earlier age at onset, higher HbA1c levels, higher frequency of Glutamic acid decarboxylase antibodies (GADA) and Insulinoma Associated-2 Autoantibodies (IA-2A) were observed in T1D patient carriers of CT genotype. The current study demonstrates that although CTLA-4-318C/T polymorphism was not linked with a higher genetic risk for T1D, the presence of a CT genotype was associated with a younger age of onset, poor control of HbA1c level and positive anti-GAD or IA-2 serum autoantibodies in Iranian Azeri population.

KEYWORDS:

Age of onset; CTLA-4; GADA; Gene polymorphism; IA-2A; Type 1 diabetes

PMID:
26051683
DOI:
10.1016/j.imlet.2015.05.021
[Indexed for MEDLINE]

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