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Proc Natl Acad Sci U S A. 2015 Jun 16;112(24):7599-604. doi: 10.1073/pnas.1413484112. Epub 2015 Jun 5.

Transgenic songbirds with suppressed or enhanced activity of CREB transcription factor.

Author information

1
Department of Biological Sciences, Graduate School of Medicine, Kyoto University, Kyoto 606-8501, Japan; Precursory Research for Embryonic Science and Technology, Japan Science and Technology Agency, Saitama 332-0012, Japan k.abe@kntrb.org dai@phy.med.kyoto-u.ac.jp.
2
Department of Biological Sciences, Graduate School of Medicine, Kyoto University, Kyoto 606-8501, Japan;
3
Department of Biological Sciences, Graduate School of Medicine, Kyoto University, Kyoto 606-8501, Japan; k.abe@kntrb.org dai@phy.med.kyoto-u.ac.jp.

Abstract

Songbirds postnatally develop their skill to utter and to perceive a vocal signal for communication. How genetic and environmental influences act in concert to regulate the development of such skill is not fully understood. Here, we report the phenotype of transgenic songbirds with altered intrinsic activity of cAMP response element-binding protein (CREB) transcription factor. By viral vector-mediated modification of genomic DNA, we established germ line-transmitted lines of zebra finches, which exhibited enhanced or suppressed activity of CREB. Although intrinsically acquired vocalizations or their hearing ability were not affected, the transgenic birds showed reduced vocal learning quality of their own songs and impaired audio-memory formation against conspecific songs. These results thus demonstrate that appropriate activity of CREB is necessary for the postnatal acquisition of learned behavior in songbirds, and the CREB transgenic birds offer a unique opportunity to separately manipulate both genetic and environmental factors that impinge on the postnatal song learning.

KEYWORDS:

CREB; postnatal development; songbird; transgenic animal; vocal learning

PMID:
26048905
PMCID:
PMC4475973
DOI:
10.1073/pnas.1413484112
[Indexed for MEDLINE]
Free PMC Article

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