Format

Send to

Choose Destination
Genome Biol. 2015 Jun 6;16:120. doi: 10.1186/s13059-015-0684-3.

Scalable microfluidics for single-cell RNA printing and sequencing.

Author information

1
Department of Systems Biology, Columbia University Medical Center, New York, NY, 10032, USA. sb3438@columbia.edu.
2
Department of Biological Sciences, Columbia University, New York, NY, 10027, USA. wanzhenmao@gmail.com.
3
Department of Biological Sciences, Columbia University, New York, NY, 10027, USA. ambrosejcarr@gmail.com.
4
Department of Biochemistry & Molecular Biophysics, Columbia University Medical Center, New York, NY, 10032, USA. abbas.rizvi@gmail.com.
5
Department of Systems Biology, Columbia University Medical Center, New York, NY, 10032, USA. gregorybv@gmail.com.
6
Department of Systems Biology, Columbia University Medical Center, New York, NY, 10032, USA. dpeer@biology.columbia.edu.
7
Department of Biological Sciences, Columbia University, New York, NY, 10027, USA. dpeer@biology.columbia.edu.
8
Department of Systems Biology, Columbia University Medical Center, New York, NY, 10032, USA. pas2182@columbia.edu.
9
Department of Biochemistry & Molecular Biophysics, Columbia University Medical Center, New York, NY, 10032, USA. pas2182@columbia.edu.
10
Sulzberger Columbia Genome Center, Columbia University Medical Center, New York, NY, 10032, USA. pas2182@columbia.edu.

Abstract

Many important biological questions demand single-cell transcriptomics on a large scale. Hence, new tools are urgently needed for efficient, inexpensive manipulation of RNA from individual cells. We report a simple platform for trapping single-cell lysates in sealed, picoliter microwells capable of printing RNA on glass or capturing RNA on beads. We then develop a scalable technology for genome-wide, single-cell RNA-Seq. Our device generates pooled libraries from hundreds of individual cells with consumable costs of $0.10-$0.20 per cell and includes five lanes for simultaneous experiments. We anticipate that this system will serve as a general platform for single-cell imaging and sequencing.

PMID:
26047807
PMCID:
PMC4487847
DOI:
10.1186/s13059-015-0684-3
[Indexed for MEDLINE]
Free PMC Article

Supplemental Content

Full text links

Icon for BioMed Central Icon for PubMed Central
Loading ...
Support Center