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Sci Rep. 2015 Jun 5;5:10932. doi: 10.1038/srep10932.

Phenotypic diversity within a Pseudomonas aeruginosa population infecting an adult with cystic fibrosis.

Author information

1
1] Department of Laboratory Medicine and Pathobiology, University of Toronto, Toronto, Canada [2] Latner Thoracic Surgery Research Laboratories, University Health Network, Toronto, Canada.
2
Department of Cell &Systems Biology, University of Toronto, Toronto, Canada.
3
Latner Thoracic Surgery Research Laboratories, University Health Network, Toronto, Canada.
4
1] Department of Cell &Systems Biology, University of Toronto, Toronto, Canada [2] Centre for the Analysis of Genome Evolution &Function, University of Toronto, Toronto, Canada.
5
Centre for the Analysis of Genome Evolution &Function, University of Toronto, Toronto, Canada.
6
1] Department of Laboratory Medicine and Pathobiology, University of Toronto, Toronto, Canada [2] Department of Pediatric Laboratory Medicine, Division of Microbiology, The Hospital for Sick Children, Toronto, Canada.
7
1] Department of Laboratory Medicine and Pathobiology, University of Toronto, Toronto, Canada [2] Department of Pediatrics, Division of Infectious Diseases, The Hospital for Sick Children, Toronto, Canada.
8
Department of Medicine, Division of Respirology, St. Michael's Hospital, Toronto, Canada.
9
1] Department of Laboratory Medicine and Pathobiology, University of Toronto, Toronto, Canada [2] Latner Thoracic Surgery Research Laboratories, University Health Network, Toronto, Canada [3] Laboratory Medicine Program, University Health Network, Toronto, Canada.

Abstract

Chronic airway infections caused by Pseudomonas aeruginosa contribute to the progression of pulmonary disease in individuals with cystic fibrosis (CF). In the setting of CF, within-patient adaptation of a P. aeruginosa strain generates phenotypic diversity that can complicate microbiological analysis of patient samples. We investigated within- and between- sample diversity of 34 phenotypes among 235 P. aeruginosa isolates cultured from sputum samples collected from a single CF patient over the span of one year, and assessed colony morphology as a screening tool for predicting phenotypes, including antimicrobial susceptibilities. We identified 15 distinct colony morphotypes that varied significantly in abundance both within and between sputum samples. Substantial within sample phenotypic heterogeneity was also noted in other phenotypes, with morphotypes being unreliable predictors of antimicrobial susceptibility and other phenotypes. Emergence of isolates with reduced susceptibility to β-lactams was observed during periods of clinical therapy with aztreonam. Our findings confirm that the P. aeruginosa population in chronic CF lung infections is highly dynamic, and that intra-sample phenotypic diversity is underestimated if only one or few colonies are analyzed per sample.

PMID:
26047320
PMCID:
PMC4456944
DOI:
10.1038/srep10932
[Indexed for MEDLINE]
Free PMC Article

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