Format

Send to

Choose Destination
Invest Ophthalmol Vis Sci. 2015 Jun;56(6):3623-34. doi: 10.1167/iovs.15-16597.

Real-Time Monitoring of Suprachoroidal Space (SCS) Following SCS Injection Using Ultra-High Resolution Optical Coherence Tomography in Guinea Pig Eyes.

Author information

1
Institute of Ocular Pharmacology, School of Ophthalmology and Optometry, Wenzhou Medical University, Wenzhou, Zhejiang, China.
2
Institute of Ocular Pharmacology, School of Ophthalmology and Optometry, Wenzhou Medical University, Wenzhou, Zhejiang, China 2Jacob's Retina Center at Shiley Eye Institute, Department of Ophthalmology, University of California San Diego, La Jolla, Califo.

Abstract

PURPOSE:

To monitor the change of suprachoroidal space (SCS) using ultra high resolution-optical coherence tomography (UHR-OCT) following SCS injection with different drug formulations.

METHODS:

An amount of 10 or 20 μL of saline or indocyanine green (ICG) or triamcinolone acetonide (TA) suspension (40 or 80 mg/mL) was injected suprachoroidally into the guinea pig eye with a 30-gauge needle. Immediately after injection, the eyes were imaged by UHR-OCT from 60 minutes up to 24 hours. At each time point, the SCS area on each OCT cross-section was measured in pixels with Image J and the area change from the baselines was analyzed over time.

RESULTS:

A 20-μL injection produced 130% to 200% SCS expansion compared to a 10-μL injection for saline and TA suspension (P < 0.01). After SCS injection, the time that expansion persisted was formulation dependent. Thus, expansion in response to injection of TA suspension, ICG, and saline persisted for 24 hours, 180 minutes, and 60 minutes, respectively. Moreover, ICG injection produced a significantly larger area of distribution in the SCS than the TA suspension (0.626 vs. 0.275 cm(2), P < 0.0001).

CONCLUSIONS:

The SCS is expandable and can recover to preinjection status after injected fluid is cleared by physiological processes. The injection-induced SCS expansion is volume-dependent, and the drug/dye retained in the SCS is formulation-dependent. The current injection technique with a volume of 20 μL or less is well tolerated in guinea pig eyes.

Comment in

PMID:
26047049
DOI:
10.1167/iovs.15-16597
[Indexed for MEDLINE]

Supplemental Content

Full text links

Icon for Silverchair Information Systems
Loading ...
Support Center