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J Gen Virol. 2015 Sep;96(9):2764-8. doi: 10.1099/vir.0.000212. Epub 2015 Jun 4.

A novel murine model for evaluating bovine papillomavirus prophylactics/therapeutics for equine sarcoid-like tumours.

Author information

1
1​Norris Comprehensive Cancer Center, University of Southern California, NRT 7507, 1450 Biggy Street, Los Angeles, CA 90033, USA 2​Department of Surgery and Anesthesiology of Domestic Animals, Faculty of Veterinary Medicine, Ghent University, Salisburylaan 133, 9820 Merelbeke, Belgium.
2
1​Norris Comprehensive Cancer Center, University of Southern California, NRT 7507, 1450 Biggy Street, Los Angeles, CA 90033, USA.
3
1​Norris Comprehensive Cancer Center, University of Southern California, NRT 7507, 1450 Biggy Street, Los Angeles, CA 90033, USA 3​Departments of Molecular Microbiology & Immunology and Obstetrics & Gynecology, University of Southern California, NRT 7507, 1450 Biggy Street, Los Angeles, CA 90033, USA.
4
2​Department of Surgery and Anesthesiology of Domestic Animals, Faculty of Veterinary Medicine, Ghent University, Salisburylaan 133, 9820 Merelbeke, Belgium.
5
4​Department of Pharmacology, Toxicology and Biochemistry, Faculty of Veterinary Medicine, Ghent University, Salisburylaan 133, 9820 Merelbeke, Belgium.
6
3​Departments of Molecular Microbiology & Immunology and Obstetrics & Gynecology, University of Southern California, NRT 7507, 1450 Biggy Street, Los Angeles, CA 90033, USA 1​Norris Comprehensive Cancer Center, University of Southern California, NRT 7507, 1450 Biggy Street, Los Angeles, CA 90033, USA.

Abstract

Equine sarcoids are highly recurrent bovine papillomavirus (BPV)-induced fibroblastic neoplasms that are the most common skin tumours in horses. In order to facilitate the study of potential equine sarcoid prophylactics or therapeutics, which can be a slow and costly process in equines, a murine model for BPV-1 protein-expressing equine sarcoid-like tumours was developed in mice through stable transfection of BPV-1 E5 and E6 in a murine fibroblast tumour cell line (K-BALB). Like equine sarcoids, these murine tumour cells (BPV-KB) were of fibroblast origin, were tumorigenic and expressed BPV-1 proteins. As an initial investigation of the preclinical potential of this tumour model for equine sarcoids prophylactics, mice were immunized with BPV-1 E5E6 Venezuelan equine encephalitis virus replicon particles, prior to BPV-KB challenge, which resulted in an increased tumour-free period compared with controls, indicating that the BPV-KB murine model may be a valuable preclinical alternative to equine clinical trials.

PMID:
26044793
PMCID:
PMC4635499
DOI:
10.1099/vir.0.000212
[Indexed for MEDLINE]
Free PMC Article

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