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Methods Mol Biol. 2015;1312:465-8. doi: 10.1007/978-1-4939-2694-7_47.

Protein detection by Simple Western™ analysis.

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Department of Pathology, University of Oklahoma Health Science Center, 940 S.L. Young Blvd., BMSB 415, Oklahoma City, OK, 73104, USA,


Protein Simple© has taken a well-known protein detection method, the western blot, and revolutionized it. The Simple Western™ system uses capillary electrophoresis to identify and quantitate a protein of interest. Protein Simple© provides multiple detection apparatuses (Wes, Sally Sue, or Peggy Sue) that are suggested to save scientists valuable time by allowing the researcher to prepare the protein sample, load it along with necessary antibodies and substrates, and walk away. Within 3-5 h the protein will be separated by size, or charge, immuno-detection of target protein will be accurately quantitated, and results will be immediately made available. Using the Peggy Sue instrument, one study recently examined changes in MAPK signaling proteins in the sex-determining stage of gonadal development. Here the methodology is described.

[Indexed for MEDLINE]

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