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Water Res. 2015 Sep 1;80:346-56. doi: 10.1016/j.watres.2015.05.001. Epub 2015 May 9.

Three stage cultivation process of facultative strain of Chlorella sorokiniana for treating dairy farm effluent and lipid enhancement.

Author information

1
School of Industrial Technology, University Sains Malaysia, George Town, Penang, 11800, Malaysia. Electronic address: sufiahena@usm.my.
2
School of Industrial Technology, University Sains Malaysia, George Town, Penang, 11800, Malaysia.
3
School of Environmental Science and Engineering, Shanghai Jiao Tong University, Shanghai, 200240, China.

Abstract

Reserve lipids of microalgae are promising for biodiesel production. However, economically feasible and sustainable energy production from microalgae requires optimization of cultivation conditions for both biomass yield and lipid production of microalgae. Biomass yield and lipid production in microalgae are a contradictory problem because required conditions for both targets are different. Simultaneously, the mass cultivation of microalgae for biofuel production also depends extremely on the performance of the microalgae strains used. In this study a green unicellular microalgae Chlorella sorokiniana (DS6) isolated from the holding tanks of farm wastewater treatment plant using multi-step screening and acclimation procedures was found high-lipid producing facultative heterotrophic microalgae strain capable of growing on dairy farm effluent (DFE) for biodiesel feedstock and wastewater treatment. Morphological features and the phylogenetic analysis for the 18S rRNA identified the isolated strains. A novel three stage cultivation process of facultative strain of C. sorokiniana was examined for lipid production.

KEYWORDS:

Chlorella sorokiniana; High cell density; Lipid production; Three stage cultivation; Triacylglycerols

PMID:
26043271
DOI:
10.1016/j.watres.2015.05.001
[Indexed for MEDLINE]

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